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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-7-7
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pubmed:abstractText |
NAD is a ubiquitous coenzyme involved in oxidation-reduction reactions and is synthesized by way of quinolinate. Animals and some bacteria synthesize quinolinate from tryptophan, whereas other bacteria synthesize quinolinate from aspartate (Asp) using L-Asp oxidase and quinolinate synthase. We show here that Arabidopsis (Arabidopsis thaliana) uses the Asp-to-quinolinate pathway. The Arabidopsis L-Asp oxidase or quinolinate synthase gene complemented the Escherichia coli mutant defective in the corresponding gene, and T-DNA-based disruption of either of these genes, as well as of the gene coding for the enzyme quinolinate phosphoribosyltransferase, was embryo lethal. An analysis of functional green fluorescent protein-fused constructs and in vitro assays of uptake into isolated chloroplasts demonstrated that these three enzymes are located in the plastid.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-10069079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-10891285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-11106374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-11198422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-12815723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-12939617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-13610899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-14700627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-14977569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-15028209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-15174128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-15595721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-16143534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-16251277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-16291748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-16339438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-16661409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-16662247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-5845278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-5935344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-6338879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-6661207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16698895-8805250
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
851-7
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pubmed:dateRevised |
2010-9-15
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
Early steps in the biosynthesis of NAD in Arabidopsis start with aspartate and occur in the plastid.
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pubmed:affiliation |
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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