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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-6-1
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pubmed:abstractText |
Interferon alpha (IFN-alpha) is produced in response to viral infections and used clinically in the therapy of a variety of cancers and viral infections. IFN-alpha treatment is often associated with severe weight reduction. To elucidate the mechanism of IFN-associated weight loss, we studied its effect on adipocytes in vitro and in vivo. Diet-induced obese (DIO) C57BL/6 mice were treated continuously for 8 days with human IFN-alpha A/D (100 U/g body weight) or with vehicle alone. The body weight and adipose cell size of IFN-alpha A/D-treated DIO mice were significantly lower (P<0.05 and P<0.001, respectively) as compared with those of control DIO mice. PI3K and Bcl-2 were down-regulated whereas Bax expression was elevated in adipose tissue following IFN treatment as compared to adipose tissue of control DIO mice. Treatment of differentiated 3T3-F442A adipocytes with IFN-alpha A/D (250 U/ml, 36 h) significantly increased the number of apoptotic cells from 15.8% in control cells to 56+/-6%. In conclusion, weight loss following IFN-alpha therapy is due at least in part to increased apoptosis of adipocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
345
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
669-74
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:16696938-3T3-L1 Cells,
pubmed-meshheading:16696938-Adipocytes,
pubmed-meshheading:16696938-Adipose Tissue,
pubmed-meshheading:16696938-Animals,
pubmed-meshheading:16696938-Antineoplastic Agents,
pubmed-meshheading:16696938-Apoptosis,
pubmed-meshheading:16696938-Body Weight,
pubmed-meshheading:16696938-Down-Regulation,
pubmed-meshheading:16696938-Humans,
pubmed-meshheading:16696938-Interferon-alpha,
pubmed-meshheading:16696938-Mice,
pubmed-meshheading:16696938-Mice, Inbred C57BL,
pubmed-meshheading:16696938-Obesity,
pubmed-meshheading:16696938-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:16696938-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:16696938-Time Factors,
pubmed-meshheading:16696938-Tumor Cells, Cultured,
pubmed-meshheading:16696938-bcl-2-Associated X Protein
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pubmed:year |
2006
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pubmed:articleTitle |
IFN-alpha induces apoptosis of adipose tissue cells.
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pubmed:affiliation |
National Institute of Biotechnology, Ben-Gurion University, Beer-Sheba 84105, Israel. rbirk@bgu.ac.il
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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