Linked Life Data

16672646

Source:http://linkedlifedata.com/resource/pubmed/id/16672646

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2006-5-4
pubmed:abstractText
2-arachidonoyl-glycerol (2-AG) is an endocannabinoid that is released from postsynaptic neurons, acts retrogradely on presynaptic cannabinoid receptor CB1, and induces short- and long-term suppression of transmitter release. To understand the mechanisms of the 2-AG-mediated retrograde modulation, we investigated subcellular localization of a major 2-AG biosynthetic enzyme, diacylglycerol lipase-alpha (DAGLalpha), by using immunofluorescence and immunoelectron microscopy in the mouse brain. In the cerebellum, DAGLalpha was predominantly expressed in Purkinje cells. DAGLalpha was detected on the dendritic surface and occasionally on the somatic surface, with a distal-to-proximal gradient from spiny branchlets toward somata. DAGLalpha was highly concentrated at the base of spine neck and also accumulated with much lower density on somatodendritic membrane around the spine neck. However, DAGLalpha was excluded from the main body of spine neck and head. In hippocampal pyramidal cells, DAGLalpha was also accumulated in spines. In contrast to the distribution in Purkinje cells, DAGLalpha was distributed in the spine head, neck, or both, whereas somatodendritic membrane was labeled very weakly. These results indicate that DAGLalpha is essentially targeted to postsynaptic spines in cerebellar and hippocampal neurons, but its fine distribution within and around spines is differently regulated between the two neurons. The preferential spine targeting should enable efficient 2-AG production on excitatory synaptic activity and its swift retrograde modulation onto nearby presynaptic terminals expressing CB1. Furthermore, different fine localization within and around spines suggests that the distance between postsynaptic 2-AG production site and presynaptic CB1 is differentially controlled depending on neuron types.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4740-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16672646-Animals, pubmed-meshheading:16672646-Animals, Newborn, pubmed-meshheading:16672646-Antibody Specificity, pubmed-meshheading:16672646-Arachidonic Acids, pubmed-meshheading:16672646-Blotting, Western, pubmed-meshheading:16672646-Brain, pubmed-meshheading:16672646-Dendritic Spines, pubmed-meshheading:16672646-Endocannabinoids, pubmed-meshheading:16672646-Glycerides, pubmed-meshheading:16672646-Guinea Pigs, pubmed-meshheading:16672646-Immunohistochemistry, pubmed-meshheading:16672646-In Situ Hybridization, pubmed-meshheading:16672646-Lipoprotein Lipase, pubmed-meshheading:16672646-Mice, pubmed-meshheading:16672646-Mice, Inbred C57BL, pubmed-meshheading:16672646-Microscopy, Immunoelectron, pubmed-meshheading:16672646-Neurons, pubmed-meshheading:16672646-Presynaptic Terminals, pubmed-meshheading:16672646-Rabbits, pubmed-meshheading:16672646-Receptor, Cannabinoid, CB1, pubmed-meshheading:16672646-Receptors, Metabotropic Glutamate, pubmed-meshheading:16672646-Vesicular Glutamate Transport Protein 2
pubmed:year
2006
pubmed:articleTitle
Localization of diacylglycerol lipase-alpha around postsynaptic spine suggests close proximity between production site of an endocannabinoid, 2-arachidonoyl-glycerol, and presynaptic cannabinoid CB1 receptor.
pubmed:affiliation
Department of Anatomy, Hokkaido University School of Medicine, Sapporo 060-8638, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't