Source:http://linkedlifedata.com/resource/pubmed/id/16668186
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
Three S-allele-associated proteins (S-proteins) of Petunia inflata, a species with gametophytic self-incompatibility, were previously found to share sequence similarity with two fungal ribonucleases, RNase T(2) and RNase Rh. In this study, the S-proteins from P. inflata plants of S(1)S(2) and S(2)S(3) genotypes were purified to homogeneity by gel filtration and cation-exchange chromatography, and their enzymatic properties were characterized. The three S-proteins (S(1), S(2), and S(3)), with pairwise sequence identity ranging from 73.1 to 80.5%, were similar in most of the enzymatic properties characterized. The ribonuclease activity had a pH optimum of 7.0 and a temperature optimum of 50 degrees C. Diethylpyrocarbonate at 1 millimolar almost completely abolished the ribonuclease activity; cupric sulfate and zinc sulfate at 1 millimolar reduced the ribonuclease activity of the three S-proteins by 50 to 75%. EDTA and RNasin had no inhibitory effect. All three S-proteins hydrolyzed polycytidylic acid preferentially, but varied in their nucleolytic activity toward polyadenylic acid and polyuridylic acid.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-16665113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-2101314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-2152130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-22021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-2237440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-2266940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-2535547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-2535548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-2594090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-5318919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-5968960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-6178316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-7176924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16668186-942051
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
96
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
61-8
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1991
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| pubmed:articleTitle |
Characterization of Ribonuclease Activity of Three S-Allele-Associated Proteins of Petunia inflata.
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| pubmed:affiliation |
Department of Molecular and Cell Biology, The Pennsylvania State University, University Park, Pennsylvania 16802.
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| pubmed:publicationType |
Journal Article
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