Linked Life Data

16667803

Source:http://linkedlifedata.com/resource/pubmed/id/16667803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-6-29
pubmed:abstractText
The effect of external inorganic carbon concentration and light on carbonic anhydrase (CA) protein accumulation and steady-state mRNA levels were examined in Chlamydomonas reinhardtii. When photoautotrophically grown high-CO(2) cells were transferred to low-CO(2) conditions, they exhibited a significant accumulation of the 2.0-kilobase CA transcript after 1 hour with the maximum level reached after 2 hours. An increase in the accumulation of the 37-kilodalton CA monomer was observed after 2-hour exposure to air. Cells allowed to adapt to air levels of CO(2) in the dark showed neither an increase in CA mRNA abundance nor in the accumulation of the enzyme. Similarly, addition of 10 micromole 3-(3,4-dichlorophenyl)-1, 1-dimethylurea immediately after transferring high-CO(2) cells to low-CO(2) condition did not cause an increase in CA transcript abundance and enzyme accumulation, suggesting that photosynthesis is absolutely required in the regulation of CA transcript abundance. In addition to the photosynthesis-dependent process, a blue light stimulated mechanism is also involved in CA transcript regulation. Experiments with transcription inhibitors confirmed the notion that the gene for carbonic anhydrase enzyme is encoded by the nuclear genome and that the induction of the enzyme depends heavily on the transcription of the CA gene.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-16593518, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-16661446, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-16663867, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-16665269, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-16666234, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-16666686, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-2112252, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-2874027, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-5157355, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-5260935, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-6315641, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-6326095, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-6356979, http://linkedlifedata.com/resource/pubmed/commentcorrection/16667803-7421598
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Nov
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1103-10
pubmed:dateRevised
2010-9-15
pubmed:year
1990
pubmed:articleTitle
Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii.
pubmed:affiliation
Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan.
pubmed:publicationType
Journal Article