pubmed-article:16666886 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:16666886 | lifeskim:mentions | umls-concept:C0007341 | lld:lifeskim |
pubmed-article:16666886 | lifeskim:mentions | umls-concept:C0036563 | lld:lifeskim |
pubmed-article:16666886 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
pubmed-article:16666886 | lifeskim:mentions | umls-concept:C0750540 | lld:lifeskim |
pubmed-article:16666886 | lifeskim:mentions | umls-concept:C0065031 | lld:lifeskim |
pubmed-article:16666886 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:16666886 | pubmed:dateCreated | 2010-6-29 | lld:pubmed |
pubmed-article:16666886 | pubmed:abstractText | The biosynthesis of nonspecific lipid transfer proteins (ns-LTPs) in germinating castor bean (Ricinus communis L.) seeds were investigated. Lipid transfer activities of ns-LTPs in the cotyledons, axis, and endosperm increased with growth after germination. The activity increases were accompanied by increased amounts of ns-LTPs in each tissue, as measured by immunoblot using anti-ns-LTP serum. These results suggest that the ns-LTPs are synthesized de novo in each tissue after germination and not activated from inactive proteins synthesized before germination. Comparison of the immunoblot products in each tissue from 4-day-old seedlings indicate the occurrence of tissue-specific isoforms of ns-LTPs; 9 kilodaltons (major) and 7 kilodaltons (minor) in the cotyledons, and 7 kilodaltons (major) and 9 kilodaltons (minor) in the axis, whereas only the 8-kilodalton ns-LTP is present in the endosperm. In vitro translation from poly(A)(+) RNAs from three tissues of castor bean seedlings and the detection of immunoprecipitated products indicate that translatable mRNAs for ns-LTPs exist in the three tissues a day before the synthesis of ns-LTPs; the translation products, which are 3.5 to 4.0 kilodaltons larger than ns-LTPs, were processed to the mature ns-LTPs. The production of mature ns-LTPs from translatable mRNAs without any delay suggests that gene expression of ns-LTPs in castor bean seedlings is controlled at a step before the formation of translatable mRNAs. | lld:pubmed |
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pubmed-article:16666886 | pubmed:language | eng | lld:pubmed |
pubmed-article:16666886 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:16666886 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
pubmed-article:16666886 | pubmed:month | Jul | lld:pubmed |
pubmed-article:16666886 | pubmed:issn | 0032-0889 | lld:pubmed |
pubmed-article:16666886 | pubmed:author | pubmed-author:WatanabeSS | lld:pubmed |
pubmed-article:16666886 | pubmed:author | pubmed-author:YamadaMM | lld:pubmed |
pubmed-article:16666886 | pubmed:author | pubmed-author:TsuboiSS | lld:pubmed |
pubmed-article:16666886 | pubmed:author | pubmed-author:OzekiYY | lld:pubmed |
pubmed-article:16666886 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:16666886 | pubmed:volume | 90 | lld:pubmed |
pubmed-article:16666886 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:16666886 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:16666886 | pubmed:pagination | 841-5 | lld:pubmed |
pubmed-article:16666886 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
pubmed-article:16666886 | pubmed:year | 1989 | lld:pubmed |
pubmed-article:16666886 | pubmed:articleTitle | Biosynthesis of nonspecific lipid transfer proteins in germinating castor bean seeds. | lld:pubmed |
pubmed-article:16666886 | pubmed:affiliation | Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan. | lld:pubmed |
pubmed-article:16666886 | pubmed:publicationType | Journal Article | lld:pubmed |
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