Source:http://linkedlifedata.com/resource/pubmed/id/16666886
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2010-6-29
|
| pubmed:abstractText |
The biosynthesis of nonspecific lipid transfer proteins (ns-LTPs) in germinating castor bean (Ricinus communis L.) seeds were investigated. Lipid transfer activities of ns-LTPs in the cotyledons, axis, and endosperm increased with growth after germination. The activity increases were accompanied by increased amounts of ns-LTPs in each tissue, as measured by immunoblot using anti-ns-LTP serum. These results suggest that the ns-LTPs are synthesized de novo in each tissue after germination and not activated from inactive proteins synthesized before germination. Comparison of the immunoblot products in each tissue from 4-day-old seedlings indicate the occurrence of tissue-specific isoforms of ns-LTPs; 9 kilodaltons (major) and 7 kilodaltons (minor) in the cotyledons, and 7 kilodaltons (major) and 9 kilodaltons (minor) in the axis, whereas only the 8-kilodalton ns-LTP is present in the endosperm. In vitro translation from poly(A)(+) RNAs from three tissues of castor bean seedlings and the detection of immunoprecipitated products indicate that translatable mRNAs for ns-LTPs exist in the three tissues a day before the synthesis of ns-LTPs; the translation products, which are 3.5 to 4.0 kilodaltons larger than ns-LTPs, were processed to the mature ns-LTPs. The production of mature ns-LTPs from translatable mRNAs without any delay suggests that gene expression of ns-LTPs in castor bean seedlings is controlled at a step before the formation of translatable mRNAs.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-3182817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-3196344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-3472236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-4212463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-6698022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-7284424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-7388001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-804327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-856266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16666886-974089
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0032-0889
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
90
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
841-5
|
| pubmed:dateRevised |
2010-9-14
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Biosynthesis of nonspecific lipid transfer proteins in germinating castor bean seeds.
|
| pubmed:affiliation |
Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan.
|
| pubmed:publicationType |
Journal Article
|