Source:http://linkedlifedata.com/resource/pubmed/id/16663839
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2010-6-29
|
| pubmed:abstractText |
The participation of pyrophosphate-dependent phosphofructokinase (PPi-PFK) in plant glycolysis was examined using extracts from pea seeds (Pisum sativum L. cv Alaska). Glycolysis starting with fructose 6-phosphate was measured under aerobic conditions as the accumulation of pyruvate. Pyruvate accumulated in a medium containing PPi and adenosine diphosphate at about two-thirds of the rate in a medium containing adenosine diphosphate and adenosine triphosphate (ATP). The PPi-dependent pyruvate accumulation had the same reactant requirements and sensitivity to glycolysis inhibitors, sodium fluoride, and iodoacetamide, as the well-established ATP-dependent glycolysis. Added fructose 2,6-bisphosphate stimulated both the PPi-dependent pyruvate accumulation and PPi-PFK activity whereas this modulator had no effect on ATP-dependent glycolysis or ATP-PFK. Collectively these results demonstrate a PPi-dependent glycolytic pathway in plants which is responsive to fructose 2,6-bisphosphate.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-13572308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-16662776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-16663136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-16663174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-171261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-219853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-4364656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-4372217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-6212061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-6297885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16663839-942051
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0032-0889
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
76
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
316-20
|
| pubmed:dateRevised |
2010-9-15
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Pyrophosphate and fructose 2,6-bisphosphate effects on glycolysis in pea seed extracts.
|
| pubmed:affiliation |
Biochemistry Department, University of Georgia, Athens, Georgia 30602.
|
| pubmed:publicationType |
Journal Article
|