| pubmed-article:16661103 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16661103 | lifeskim:mentions | umls-concept:C0040329 | lld:lifeskim |
| pubmed-article:16661103 | lifeskim:mentions | umls-concept:C0033731 | lld:lifeskim |
| pubmed-article:16661103 | lifeskim:mentions | umls-concept:C0042219 | lld:lifeskim |
| pubmed-article:16661103 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:16661103 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:16661103 | lifeskim:mentions | umls-concept:C0728938 | lld:lifeskim |
| pubmed-article:16661103 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:16661103 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16661103 | pubmed:dateCreated | 2010-6-29 | lld:pubmed |
| pubmed-article:16661103 | pubmed:abstractText | Protoplasts from suspension-cultured cells of Nicotiana glutinosa L. were lysed in 0.3 molar sorbitol in 2 millimolar ethylenediaminetetraacetate-tris(hydroxymethyl) aminomethane (pH 7.5) to release intact vacuoles. The vacuoles were purified by centrifugation in a Ficoll step gradient. About 11% of the vacuoles and 13% of the acid phosphatase activity was recovered in the purified vacuole fraction, suggesting that the vacuole is the major site for acid phosphatase in these cells. NADH-cytochrome c reductase, malate dehydrogenase, and cytochrome c oxidase activities were reduced during vacuole purification. The majority of the adenosine 5'-triphosphate (ATP) hydrolytic activity of purified vacuoles was associated with nonspecific acid phosphatase and not with a transport ATPase. As judged by acid phosphatase distribution and electron microscopy, the effective density of vacuoles in a sucrose gradient was low (less than 1.1 grams per cubic centimeter), although an unequivocal estimate of the vacuole or tonoplast density was not possible from the experiments conducted. | lld:pubmed |
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| pubmed-article:16661103 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16661103 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16661103 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16661103 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16661103 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16661103 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16661103 | pubmed:status | PubMed-not-MEDLINE | lld:pubmed |
| pubmed-article:16661103 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16661103 | pubmed:issn | 0032-0889 | lld:pubmed |
| pubmed-article:16661103 | pubmed:author | pubmed-author:LeonardR TRT | lld:pubmed |
| pubmed-article:16661103 | pubmed:author | pubmed-author:MettlerI JIJ | lld:pubmed |
| pubmed-article:16661103 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16661103 | pubmed:volume | 64 | lld:pubmed |
| pubmed-article:16661103 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16661103 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16661103 | pubmed:pagination | 1114-20 | lld:pubmed |
| pubmed-article:16661103 | pubmed:dateRevised | 2010-9-15 | lld:pubmed |
| pubmed-article:16661103 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:16661103 | pubmed:articleTitle | Isolation and partial characterization of vacuoles from tobacco protoplasts. | lld:pubmed |
| pubmed-article:16661103 | pubmed:affiliation | Department of Botany and Plant Sciences, University of California, Riverside, California 92521. | lld:pubmed |
| pubmed-article:16661103 | pubmed:publicationType | Journal Article | lld:pubmed |
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