Source:http://linkedlifedata.com/resource/pubmed/id/16661103
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2010-6-29
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| pubmed:abstractText |
Protoplasts from suspension-cultured cells of Nicotiana glutinosa L. were lysed in 0.3 molar sorbitol in 2 millimolar ethylenediaminetetraacetate-tris(hydroxymethyl) aminomethane (pH 7.5) to release intact vacuoles. The vacuoles were purified by centrifugation in a Ficoll step gradient. About 11% of the vacuoles and 13% of the acid phosphatase activity was recovered in the purified vacuole fraction, suggesting that the vacuole is the major site for acid phosphatase in these cells. NADH-cytochrome c reductase, malate dehydrogenase, and cytochrome c oxidase activities were reduced during vacuole purification. The majority of the adenosine 5'-triphosphate (ATP) hydrolytic activity of purified vacuoles was associated with nonspecific acid phosphatase and not with a transport ATPase. As judged by acid phosphatase distribution and electron microscopy, the effective density of vacuoles in a sucrose gradient was low (less than 1.1 grams per cubic centimeter), although an unequivocal estimate of the vacuole or tonoplast density was not possible from the experiments conducted.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-13830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16591795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16658403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16658500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16659414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16659673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16659738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16660001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16660045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16660441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16660675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-16660869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16661103-4608754
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Dec
|
| pubmed:issn |
0032-0889
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
64
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1114-20
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| pubmed:dateRevised |
2010-9-15
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| pubmed:year |
1979
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| pubmed:articleTitle |
Isolation and partial characterization of vacuoles from tobacco protoplasts.
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| pubmed:affiliation |
Department of Botany and Plant Sciences, University of California, Riverside, California 92521.
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| pubmed:publicationType |
Journal Article
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