Source:http://linkedlifedata.com/resource/pubmed/id/16653231
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2010-6-29
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pubmed:abstractText |
The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formation of protoplast chains in the presence of CaCl(2) up to 5 mm, and protoplast fusion in the presence of 3 mm CaCl(2) were found when the pulse field was applied. The present results suggest the possibility of electrically induced protoplast fusion at cation concentrations that prevent fusion when sine-wave fields are applied.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-16663128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-16664564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-16665279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-2369580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-3778446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-5132497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-6561138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-6733258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-6758848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16653231-7050391
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pubmed:language |
eng
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pubmed:journal | |
pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0032-0889
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2008-12
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pubmed:dateRevised |
2010-9-14
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pubmed:year |
1992
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pubmed:articleTitle |
Electrically induced protoplast fusion using pulse electric fields for dielectrophoresis.
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pubmed:affiliation |
Bulgarian Academy of Sciences, Institute of Plant Physiology, Acad. G. Bonchev str., bl 21, 1113 Sofia, Bulgaria.
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pubmed:publicationType |
Journal Article
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