Linked Life Data

1664433

Source:http://linkedlifedata.com/resource/pubmed/id/1664433

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-3-19
pubmed:abstractText
An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to BLV in milk and serum (Juntti et al., 1989). The conjugate consists of a monoclonal anti-bovine IgG1 and IgG2 labelled with horseradish peroxidase (HRP). The indirect ELISA was calibrated with EEC reference serum E 4. Standard serum E 4 was scored positive when diluted 8192 times in negative milk and between 12,800 and 25,600 times in negative serum. The sensitivity and specificity of the indirect ELISA relative to the agar gel immunodiffusion test (AGID) were 100% and 99.8%, respectively. ELISA results for milk and sera from 614 dairy cows agreed to 100%. The absorbance value in bulk milk could be used to roughly predict the rate of BLV infection among lactating cows in a herd. An infection rate of 4 to 5% in a herd could be detected in the ELISA. Results were applied in a nation-wide screening of more than 24,000 bulk-milk samples, and the subsequent introduction of an eradication programme for BLV. The aim is to eliminate the infection from Swedish herds in 5 to 10 years.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
319-33
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Evaluation of an indirect ELISA for the detection of antibodies to bovine leukaemia virus in milk and serum.
pubmed:affiliation
Department of Virology, National Veterinary Institute, Uppsala, Sweden.
pubmed:publicationType
Journal Article