Linked Life Data

1663725

Source:http://linkedlifedata.com/resource/pubmed/id/1663725

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-3-5
pubmed:abstractText
Phosphonylmethoxyalkylpurines and -pyrimidines, particularly (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (HPMPC) and (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) and their cyclic forms (cHPMPC, cHPMPA) and the 3-deaza analogue of HPMPA (HPMPc3A) are selective and potent inhibitors of human cytomegalovirus (CMV) replication in vitro. Their anti-CMV activity has been monitored by flow cytometry and DNA hybridization. The anti-CMV agent ganciclovir [9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG)] was included as a reference compound. From the flow cytometric assays, HPMPC and HPMPA were the most active compounds, with an EC50 (50% effective concentration) of approximately 0.6 microM. The EC50 values obtained by DNA hybridization ranged from 0.05 microM (HPMPC) to 0.74 microM (DHPG). Selectivity indexes, calculated as the ratio of the 50% inhibitory concentration (CC50) for cell growth or [methyl-3H]-thymidine incorporation to the EC50 for virus replication were highest for HPMPC and its cyclic derivative (cHPMPC). The flow cytometry and DNA hybridization assays thus confirm the results obtained by the classic plaque assay. They allow a quantitative estimation of the anti-CMV potency of the test compounds.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0166-3542
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Antiviral activity of anti-cytomegalovirus agents (HPMPC, HPMPA) assessed by a flow cytometric method and DNA hybridization technique.
pubmed:affiliation
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't