| pubmed-article:16636015 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16636015 | lifeskim:mentions | umls-concept:C0026926 | lld:lifeskim |
| pubmed-article:16636015 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:16636015 | lifeskim:mentions | umls-concept:C0054871 | lld:lifeskim |
| pubmed-article:16636015 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:16636015 | lifeskim:mentions | umls-concept:C0221099 | lld:lifeskim |
| pubmed-article:16636015 | lifeskim:mentions | umls-concept:C1254042 | lld:lifeskim |
| pubmed-article:16636015 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:16636015 | pubmed:dateCreated | 2006-5-29 | lld:pubmed |
| pubmed-article:16636015 | pubmed:abstractText | Mycobacterium tuberculosis-infected macrophages demonstrate diminished capacity to present antigens via class II MHC molecules. Since successful class II MHC-restricted antigen presentation relies on the actions of endocytic proteases, we asked whether the activities of cathepsins (Cat) B, S and L-three major lysosomal cysteine proteases-are modulated in macrophages infected with pathogenic Mycobacterium spp. Infection of murine bone marrow-derived macrophages with either Mycobacterium avium or M. tuberculosis had no obvious effect on Cat B or Cat S activity. In contrast, the activity of Cat L was altered in infected cells. Specifically, whereas the 24-kDa two-chain mature form of active Cat L predominated in uninfected cells, we observed an increase in the steady-state activity of the precursor single-chain (30 kDa) and 25-kDa two-chain forms of the enzyme in cells infected with either M. avium or M. tuberculosis. Pulse-chase analyses revealed that maturation of nascent, single-chain Cat L into the 25-kDa two-chain form was impaired in infected macrophages, and that maturation into the 24-kDa two-chain form did not occur. Consistent with these data, M. avium infection inhibited the IFNgamma-induced secretion of active two-chain Cat L by macrophages. Viable bacilli were not required to disrupt Cat L maturation, suggesting that a constitutively expressed mycobacterial component was responsible. The absence of the major active form of lysosomal Cat L in M. avium- and M. tuberculosis-infected macrophages may influence the types of T cell epitopes generated in these antigen-presenting cells, and/or the rate of class II MHC peptide loading. | lld:pubmed |
| pubmed-article:16636015 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16636015 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16636015 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16636015 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:16636015 | pubmed:issn | 0953-8178 | lld:pubmed |
| pubmed-article:16636015 | pubmed:author | pubmed-author:EricksonAnn... | lld:pubmed |
| pubmed-article:16636015 | pubmed:author | pubmed-author:BryantPaulaP | lld:pubmed |
| pubmed-article:16636015 | pubmed:author | pubmed-author:ShafferBrianB | lld:pubmed |
| pubmed-article:16636015 | pubmed:author | pubmed-author:NepalRajeev... | lld:pubmed |
| pubmed-article:16636015 | pubmed:author | pubmed-author:MampeStephani... | lld:pubmed |
| pubmed-article:16636015 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16636015 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:16636015 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16636015 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16636015 | pubmed:pagination | 931-9 | lld:pubmed |
| pubmed-article:16636015 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
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| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:meshHeading | pubmed-meshheading:16636015... | lld:pubmed |
| pubmed-article:16636015 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16636015 | pubmed:articleTitle | Cathepsin L maturation and activity is impaired in macrophages harboring M. avium and M. tuberculosis. | lld:pubmed |
| pubmed-article:16636015 | pubmed:affiliation | Department of Microbiology, Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA. | lld:pubmed |
| pubmed-article:16636015 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16636015 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:13030 | entrezgene:pubmed | pubmed-article:16636015 | lld:entrezgene |
| entrez-gene:13040 | entrezgene:pubmed | pubmed-article:16636015 | lld:entrezgene |
