Source:http://linkedlifedata.com/resource/pubmed/id/16631357
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2006-8-22
|
| pubmed:abstractText |
Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAcalpha1-3 (Fucalpha1-2) Gal-: A-trisaccharide] expressed in the intestinal mucosa. Eleven strains showed strong adherence to an A-trisaccharide biotinyl polymer (BP) probe, and slightly or no adherence to a B-trisaccharide BP probe. Four strains with high adherence (high A/B ratio) were selected and their surface layer proteins (SLPs) were evaluated for A-antigen ligand binding using BIACORE. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa by SDS-PAGE analysis and it had a very strong adherence to the human A-antigen, as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed strong homology to an S-layer protein of L. brevis ATCC8287T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0923-2508
|
| pubmed:author |
pubmed-author:HoriiAkiraA,
pubmed-author:KawaiYasushiY,
pubmed-author:KimuraKatsunoriK,
pubmed-author:KinoshitaHidekiH,
pubmed-author:KitazawaHarukiH,
pubmed-author:MiuraKohK,
pubmed-author:OdaMunehiroM,
pubmed-author:SaitoTadaoT,
pubmed-author:ShiibaKenichiK,
pubmed-author:TaketomoNaokiN,
pubmed-author:UchidaHideakiH,
pubmed-author:YajimaTakajiT
|
| pubmed:issnType |
Print
|
| pubmed:volume |
157
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
659-65
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:16631357-ABO Blood-Group System,
pubmed-meshheading:16631357-Bacterial Adhesion,
pubmed-meshheading:16631357-Bacterial Proteins,
pubmed-meshheading:16631357-Biosensing Techniques,
pubmed-meshheading:16631357-Feces,
pubmed-meshheading:16631357-Humans,
pubmed-meshheading:16631357-Intestinal Mucosa,
pubmed-meshheading:16631357-Lactobacillus,
pubmed-meshheading:16631357-Protein Binding,
pubmed-meshheading:16631357-Surface Plasmon Resonance
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Lactobacilli binding human A-antigen expressed in intestinal mucosa.
|
| pubmed:affiliation |
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Miyagi, Japan. hideaki7@bios.tohoku.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|