Linked Life Data

16604466

Source:http://linkedlifedata.com/resource/pubmed/id/16604466

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-4-10
pubmed:abstractText
A number of quantitative, real-time PCR methods have been developed for determining transgene copy numbers in plants. Here, we demonstrate that the Roche LightCycler system can be used to determine the zygosity of transgenic lines without the use of standard curves or efficiency correction calculations. We have developed a duplex PCR assay which permits the determination of zygosity, relative to a calibrator sample, in transgenic rice lines containing the gene for a viral glycoprotein. Our data demonstrate that unambiguous 2-fold discrimination of copy number can be attained by calculating relative copy number using the threshold crossing point (Ct) calculated by the LightCycler software combined with delta delta Ct calculations, provided that the appropriate calibrator sample is included in each run. The method presented here is rapid, sensitive, robust and easy to optimise.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0962-8819
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-5
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Accurate determination of zygosity in transgenic rice by real-time PCR does not require standard curves or efficiency correction.
pubmed:affiliation
Centre for Biologics Research, Biologics and Genetic Therapies Directorate, Health Canada, Sir Frederick Banting Research Centre, Tunney's Pasture, Ottawa, Ontario.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't