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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2010-6-30
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pubmed:abstractText |
We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus harboring the foreign gene was recovered from the bombarded tissue by selection on medium containing kanamycin. Kanamycin-resistant plants have subsequently been regenerated from the callus derived from leaves. Transient expression of an introduced beta-glucuronidase gene was used to assess the efficiency of DNA delivery by microprojectiles. The frequency of cells that were stably transformed with the neomycin phosphotransferase gene was a few percent of the cells that transiently expressed the beta-glucuronidase gene. These results show that gene transfer by high-velocity microprojectiles is a rapid and direct means for transforming intact plant cells and tissues that eliminates the need for production of protoplasts or infection by Agrobacterium.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-16453538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-16453573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-16593942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-17759205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-17770329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-17801640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-2832947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-3005872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-3016708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-3163765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-3323803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-3327686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-3411404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-3519133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-5650857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-7153687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593993-7363328
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pubmed:language |
eng
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pubmed:journal |
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pubmed:status |
PubMed-not-MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8502-5
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pubmed:dateRevised |
2010-9-15
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pubmed:year |
1988
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pubmed:articleTitle |
Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.
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pubmed:affiliation |
Plant Gene Expression Center, U.S. Department of Agriculture, Agricultural Research Service, 800 Buchanan Street, Albany, CA 94710.
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pubmed:publicationType |
Journal Article
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