Source:http://linkedlifedata.com/resource/pubmed/id/16593964
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
15
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| pubmed:dateCreated |
2010-6-30
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| pubmed:abstractText |
Culture conditions were developed that induce Arabidopsis thaliana (L.) Heynh. root cuttings to regenerate shoots rapidly and at 100% efficiency. The shoots produce viable seeds in vitro or after rooting in soil. A transformation procedure for Arabidopsis root explants based on kanamycin selection was established. By using this regeneration procedure and an Agrobacterium tumor-inducing Ti plasmid carrying a chimeric neomycin phosphotransferase II gene (neo), transformed seed-producing plants were obtained with an efficiency between 20% and 80% within 3 months after gene transfer. F(1) seedlings of these transformants showed Mendelian segregation of the kanamycin-resistance trait. The transformation method could be applied to three different Arabidopsis ecotypes. In addition to the neo gene, a chimeric bar gene conferring resistance to the herbicide Basta was introduced into Arabidopsis. The expression of the bar gene was shown by enzymatic assay.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-16453789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-16664795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-17792019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-2830671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-3327474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-3534794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-4022773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-4059052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-4858229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-5650857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-6096215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-6825936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16593964-804998
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0027-8424
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
85
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5536-40
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| pubmed:dateRevised |
2010-9-14
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| pubmed:year |
1988
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| pubmed:articleTitle |
Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection.
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| pubmed:affiliation |
Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Ghent, Belgium.
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| pubmed:publicationType |
Journal Article
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