Linked Life Data

1656593

Source:http://linkedlifedata.com/resource/pubmed/id/1656593

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-11-21
pubmed:abstractText
We have constructed a HSV-1 UL45 null mutant (UL45 delta) by inserting a TK-lacZ cassette into a BclI site near the 5' end of the UL45 gene. A polyclonal antiserum produced to an Escherichia coli trpE:UL45 fusion protein was used to show that an 18-kDa polypeptide corresponding to the predicted UL45 gene product was produced in HSV-1 strain KOS-infected Vero cells but was not detected in UL45 delta-infected Vero cells. The absence of the 18-kDa protein had only a slight effect on viral growth in cell culture, indicating that the UL45 gene product is not essential for growth in Vero cells. However, the burst size of UL45 delta was smaller than HSV-1 KOS in Vero and HeLa cells. UL45 delta also had a smaller plaque size and an altered plaque morphology.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
185
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
419-23
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
The HSV-1 UL45 gene product is not required for growth in Vero cells.
pubmed:affiliation
Department of Medical Microbiology/Immunology, University of Wisconsin, Madison 53706.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't