Source:http://linkedlifedata.com/resource/pubmed/id/16555281
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2006-3-28
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| pubmed:abstractText |
Human metapneumovirus (hMPV) is a newly identified paramixovirus, associated with respiratory illnesses in all age groups. Two genetic groups of hMPV have been described. The nucleotide sequences of the G and F genes from 11 Argentinean hMPV strains (1998-2003) were determined by RT-PCR and direct sequencing. Phylogenetic analysis showed that hMPV strains clustered into two main genetic lineages, A and B. Strains clustered into A group were split into two sublineages, A1 and A2. All strains belonging to group B clustered with representative strains from sublineage B1. No Argentinean strains belonged to sublineage B2. F sequences showed high percentage identities at nucleotide and amino acid levels. In contrast, G sequences showed high diversity between A and B groups. Most changes observed in the deduced G protein sequence were amino acid substitutions in the extracellular domain, and changes in stop codon usage leading to different lengths in the G proteins. High content of serine and threonine residues were also shown, suggesting that this protein would be highly glycosylated. The potential sites for N- and O-glycosylation seem to have a different conservation pattern between the two main groups. This is the first report on the genetic variability of the G and F protein genes of hMPV strains in South America. Two main genetic groups and at least three subgroups were revealed among Argentinean hMPV strains. The F protein seems to be highly conserved, whereas the G protein showed extensive diversity between groups A and B.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0146-6615
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2006 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
78
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
631-7
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:16555281-Amino Acid Sequence,
pubmed-meshheading:16555281-Amino Acid Substitution,
pubmed-meshheading:16555281-Argentina,
pubmed-meshheading:16555281-Child, Preschool,
pubmed-meshheading:16555281-Consensus Sequence,
pubmed-meshheading:16555281-Genes, Viral,
pubmed-meshheading:16555281-Genetic Variation,
pubmed-meshheading:16555281-Glycoproteins,
pubmed-meshheading:16555281-Humans,
pubmed-meshheading:16555281-Infant,
pubmed-meshheading:16555281-Metapneumovirus,
pubmed-meshheading:16555281-Molecular Sequence Data,
pubmed-meshheading:16555281-Paramyxoviridae Infections,
pubmed-meshheading:16555281-Polymerase Chain Reaction,
pubmed-meshheading:16555281-Sequence Alignment,
pubmed-meshheading:16555281-Species Specificity,
pubmed-meshheading:16555281-Viral Fusion Proteins,
pubmed-meshheading:16555281-Viral Proteins
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Genetic heterogeneity of G and F protein genes from Argentinean human metapneumovirus strains.
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| pubmed:affiliation |
Laboratorio de Virología Clínica, Centro de Educación Médica e Investigaciones Clínicas, CEMIC, Hospital Universitario, Buenos Aires, Argentina.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|