Source:http://linkedlifedata.com/resource/pubmed/id/16554442
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 7
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| pubmed:dateCreated |
2006-3-23
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| pubmed:abstractText |
Post-transcriptional regulation exerted by neural-specific RNA-binding proteins plays a pivotal role in the development and maintenance of the nervous system. Neural ELAV proteins are key inducers of neuronal differentiation through the stabilization and/or translational enhancement of target transcripts bearing the AU-rich elements (AREs), whereas Musashi-1 maintains the stem cell proliferation state by acting as a translational repressor. Since the gene encoding Musashi-1 (Msi1) contains a conserved ARE in its 3' untranslated region, we focused on the possibility of a mechanistic relationship between ELAV proteins and Musashi-1 in cell fate commitment. Colocalization of neural ELAV proteins with Musashi-1 clearly shows that ELAV proteins are expressed at early stages of neural commitment, whereas interaction studies demonstrate that neural ELAV proteins exert an ARE-dependent binding activity on the Msi1 mRNA. This binding activity has functional effects, since the ELAV protein family member HuD is able to stabilize the Msi1 ARE-containing mRNA in a sequence-dependent way in a deadenylation/degradation assay. Furthermore activation of the neural ELAV proteins by phorbol esters in human SH-SY5Y cells is associated with an increase of Musashi-1 protein content in the cytoskeleton. We propose that ELAV RNA-binding proteins exert an important post-transcriptional control on Musashi-1 expression in the transition from proliferation to neural differentiation of stem/progenitor cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Hu Paraneoplastic...,
http://linkedlifedata.com/resource/pubmed/chemical/Msi1h protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0021-9533
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
119
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1442-52
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16554442-Amino Acid Sequence,
pubmed-meshheading:16554442-Animals,
pubmed-meshheading:16554442-Base Sequence,
pubmed-meshheading:16554442-Brain,
pubmed-meshheading:16554442-Cells, Cultured,
pubmed-meshheading:16554442-Hu Paraneoplastic Encephalomyelitis Antigens,
pubmed-meshheading:16554442-Immunohistochemistry,
pubmed-meshheading:16554442-Mice,
pubmed-meshheading:16554442-Mice, Inbred Strains,
pubmed-meshheading:16554442-Models, Biological,
pubmed-meshheading:16554442-Molecular Sequence Data,
pubmed-meshheading:16554442-Nerve Tissue Proteins,
pubmed-meshheading:16554442-Neurons,
pubmed-meshheading:16554442-Precipitin Tests,
pubmed-meshheading:16554442-RNA, Messenger,
pubmed-meshheading:16554442-RNA Stability,
pubmed-meshheading:16554442-RNA-Binding Proteins,
pubmed-meshheading:16554442-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16554442-Sequence Homology, Amino Acid,
pubmed-meshheading:16554442-Sequence Homology, Nucleic Acid,
pubmed-meshheading:16554442-Stem Cells
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| pubmed:year |
2006
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| pubmed:articleTitle |
A role for the ELAV RNA-binding proteins in neural stem cells: stabilization of Msi1 mRNA.
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| pubmed:affiliation |
Department of Neuroscience, Dino Ferrari Centre, University of Milan-IRCCS Istituto Auxologico Italiano, Via Zucchi 18, 20095 Cusano Milanino, Italy. antonia.ratti@unimi.it
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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