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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7089
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pubmed:dateCreated |
2006-5-4
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pubmed:abstractText |
Segregation of homologous maternal and paternal centromeres to opposite poles during meiosis I depends on post-replicative crossing over between homologous non-sister chromatids, which creates chiasmata and therefore bivalent chromosomes. Destruction of sister chromatid cohesion along chromosome arms due to proteolytic cleavage of cohesin's Rec8 subunit by separase resolves chiasmata and thereby triggers the first meiotic division. This produces univalent chromosomes, the chromatids of which are held together by centromeric cohesin that has been protected from separase by shugoshin (Sgo1/MEI-S332) proteins. Here we show in both fission and budding yeast that Sgo1 recruits to centromeres a specific form of protein phosphatase 2A (PP2A). Its inactivation causes loss of centromeric cohesin at anaphase I and random segregation of sister centromeres at the second meiotic division. Artificial recruitment of PP2A to chromosome arms prevents Rec8 phosphorylation and hinders resolution of chiasmata. Our data are consistent with the notion that efficient cleavage of Rec8 requires phosphorylation of cohesin and that this is blocked by PP2A at meiosis I centromeres.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoprotein Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 2,
http://linkedlifedata.com/resource/pubmed/chemical/REC8 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/REC8 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Schizosaccharomyces pombe Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sgo1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Sgo1 protein, S pombe,
http://linkedlifedata.com/resource/pubmed/chemical/cohesins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1476-4687
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pubmed:author |
pubmed-author:BuchholzFrankF,
pubmed-author:CetinBulentB,
pubmed-author:GálováMartaM,
pubmed-author:GreganJurajJ,
pubmed-author:HelmhartWolfgangW,
pubmed-author:ItohTakehikoT,
pubmed-author:KatisVittorio LVL,
pubmed-author:KatouYukiY,
pubmed-author:MechtlerKarlK,
pubmed-author:MoriSaoriS,
pubmed-author:MudrakIngridI,
pubmed-author:NasmythKimK,
pubmed-author:OgrisEgonE,
pubmed-author:PelletierLaurenceL,
pubmed-author:PetronczkiMarkM,
pubmed-author:RiedelChristian GCG,
pubmed-author:ShirahigeKatsuhikoK
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
441
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-61
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16541024-Animals,
pubmed-meshheading:16541024-Cell Cycle Proteins,
pubmed-meshheading:16541024-Cell Line,
pubmed-meshheading:16541024-Centromere,
pubmed-meshheading:16541024-Chromatids,
pubmed-meshheading:16541024-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:16541024-Chromosome Pairing,
pubmed-meshheading:16541024-Conserved Sequence,
pubmed-meshheading:16541024-Humans,
pubmed-meshheading:16541024-Meiosis,
pubmed-meshheading:16541024-Mice,
pubmed-meshheading:16541024-Nuclear Proteins,
pubmed-meshheading:16541024-Phosphoprotein Phosphatases,
pubmed-meshheading:16541024-Phosphoproteins,
pubmed-meshheading:16541024-Phosphorylation,
pubmed-meshheading:16541024-Protein Binding,
pubmed-meshheading:16541024-Protein Phosphatase 2,
pubmed-meshheading:16541024-Saccharomyces cerevisiae,
pubmed-meshheading:16541024-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:16541024-Schizosaccharomyces,
pubmed-meshheading:16541024-Schizosaccharomyces pombe Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.
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pubmed:affiliation |
Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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