Source:http://linkedlifedata.com/resource/pubmed/id/16530175
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2006-4-20
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pubmed:abstractText |
The enzymatic glucosylation of luteolin was attempted using two glucansucrases: the dextransucrase from Leuconostoc mesenteroides NRRL B-512F and the alternansucrase from L. mesenteroides NRRL B-23192. Reactions were carried out in aqueous-organic solvents to improve luteolin solubility. A molar conversion of 44% was achieved after 24h of reaction catalysed by dextransucrase from L. mesenteroides NRRL B-512F in a mixture of acetate buffer (70%)/bis(2-methoxyethyl) ether (30%). Two products were characterised by nuclear magnetic resonance (NMR) spectroscopy: luteolin-3'-O-alpha-d-glucopyranoside and luteolin-4'-O-alpha-d-glucopyranoside. In the presence of alternansucrase from L. mesenteroides NRRL B-23192, three additional products were obtained with a luteolin conversion of 8%. Both enzymes were also able to glucosylate quercetin and myricetin with conversion of 4% and 49%, respectively.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Glucosides,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Solvents,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/alternansucrase
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0008-6215
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
341
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
855-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16530175-Buffers,
pubmed-meshheading:16530175-Glucosides,
pubmed-meshheading:16530175-Glycosyltransferases,
pubmed-meshheading:16530175-Leuconostoc,
pubmed-meshheading:16530175-Molecular Conformation,
pubmed-meshheading:16530175-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:16530175-Solubility,
pubmed-meshheading:16530175-Solvents,
pubmed-meshheading:16530175-Substrate Specificity,
pubmed-meshheading:16530175-Water
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pubmed:year |
2006
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pubmed:articleTitle |
Leuconostoc mesenteroides glucansucrase synthesis of flavonoid glucosides by acceptor reactions in aqueous-organic solvents.
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pubmed:affiliation |
Laboratoire Biotechnologie-Bioprocédés UMR CNRS 5504, UMR INRA 792, INSA DGBA, 135 avenue de Rangueil, 31077 Toulouse Cedex 04, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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