Source:http://linkedlifedata.com/resource/pubmed/id/16517010
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
2006-6-19
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pubmed:abstractText |
Activation of natriuretic peptide receptor-A (NPRA) produces the second messenger cGMP, which plays a pivotal role in maintaining blood pressure and cardiovascular homeostasis. In the present study, we have examined the role of trans-acting factor Ets-1 in transcriptional regulation of Npr1 gene (coding for NPRA). Using deletional analysis of the Npr1 promoter, we have defined a 400 base pair (bp) region as the core promoter, which contains consensus binding sites for transcription factors including: Ets-1, Lyf-1, and GATA-1/2. Overexpression of Ets-1 in mouse mesangial cells (MMCs) enhanced Npr1 gene transcription by 12-fold. However, overexpression of GATA-1 or Lyf-1 repressed Npr1 basal promoter activity by 50% and 80%, respectively. The constructs having a mutant Ets-1 binding site or lacking this site failed to respond to Ets-1 activation of Npr1 gene transcription. Collectively, the present results demonstrate that Ets-1 greatly stimulates Npr1 gene promoter activity, implicating its critical role in the regulation and function of NPRA at the molecular level.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Protein c-ets-1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Atrial Natriuretic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/atrial natriuretic factor receptor A
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0196-9781
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1762-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16517010-Animals,
pubmed-meshheading:16517010-Base Sequence,
pubmed-meshheading:16517010-Binding Sites,
pubmed-meshheading:16517010-Blood Pressure,
pubmed-meshheading:16517010-Cell Nucleus,
pubmed-meshheading:16517010-Gene Expression Regulation,
pubmed-meshheading:16517010-Guanylate Cyclase,
pubmed-meshheading:16517010-Mesangial Cells,
pubmed-meshheading:16517010-Mice,
pubmed-meshheading:16517010-Molecular Sequence Data,
pubmed-meshheading:16517010-Mutagenesis, Site-Directed,
pubmed-meshheading:16517010-Plasmids,
pubmed-meshheading:16517010-Proto-Oncogene Protein c-ets-1,
pubmed-meshheading:16517010-Receptors, Atrial Natriuretic Factor,
pubmed-meshheading:16517010-Transcription, Genetic
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pubmed:year |
2006
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pubmed:articleTitle |
Transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-A gene.
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pubmed:affiliation |
Department of Physiology, SL-39, Tulane University Health Sciences Center and School of Medicine, 1430 Tulane Ave, New Orleans, LA 70112, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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