Linked Life Data

1651571

Source:http://linkedlifedata.com/resource/pubmed/id/1651571

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1991-9-18
pubmed:abstractText
Gallium arsenide (GaAs) has been shown previously to suppress the in vivo antibody-forming cell (AFC) response to sheep erythrocytes (SRBC) when administered intratracheally at concentrations between 50 and 200 mg/kg. In the present studies, direct addition of GaAs to in vitro-generated antibody cultures resulted in dose-dependent suppression of the primary antibody response, and was only seen when GaAs was added within 36 hr following immunization. Using atomic absorption spectrophotometry on tissue samples from mice exposed to 200 mg/kg GaAs, arsenic concentrations were found to peak in the spleen at 24 hr and decline, whereas gallium concentrations continue to rise through 14 days. Concentrations of each metal in the spleen at 24 hr are comparable to the concentrations achieved for each metal when GaAs is added at 25 microM to the in vitro model system. The 24 hr time point was chosen for comparison because all in vivo-in vitro studies were conducted using spleens from mice 24 hr after GaAs exposure. NaAsO2 and Ga(NO3)3 suppressed the AFC response dose-dependently, and in a time-dependent manner similar to GaAs when added to the in vitro system. However, based on IC50 values for each salt, the role of the gallium component in the immunosuppression appears weak. Oxalic acid (OA) and meso-2,3-dimercaptosuccinic acid (DMSA), chelators of gallium and arsenic respectively, were added to cultures with GaAs to confirm that arsenic was the primary immunosuppressive component. DMSA dose-dependently blocked GaAs-induced immunosuppression in vitro, while OA had no effect. The metal-binding compounds were determined to be specific for the metals used in these studies and did not cross-react with one another. DMSA was evaluated for its ability to prevent suppression of the AFC response in splenocytes from GaAs-exposed mice and was able to block GaAs-induced suppression of the AFC response when given sc every 4 hr beginning 1 hr prior to GaAs exposure. These data indicate that the arsenic component of GaAs is the major contributor to the GaAs-induced immunosuppression and that this effect occurs within the first 36 hr of the 5-day culture period in a concentration-dependent manner.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
110
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-69
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1651571-Animals, pubmed-meshheading:1651571-Antibody Formation, pubmed-meshheading:1651571-Arsenic, pubmed-meshheading:1651571-Arsenicals, pubmed-meshheading:1651571-Arsenites, pubmed-meshheading:1651571-Cells, Cultured, pubmed-meshheading:1651571-Dose-Response Relationship, Drug, pubmed-meshheading:1651571-Female, pubmed-meshheading:1651571-Gallium, pubmed-meshheading:1651571-Immune Tolerance, pubmed-meshheading:1651571-Immunoglobulin M, pubmed-meshheading:1651571-Mice, pubmed-meshheading:1651571-Models, Biological, pubmed-meshheading:1651571-Oxalates, pubmed-meshheading:1651571-Oxalic Acid, pubmed-meshheading:1651571-Sodium Compounds, pubmed-meshheading:1651571-Spectrophotometry, Atomic, pubmed-meshheading:1651571-Spleen, pubmed-meshheading:1651571-Succimer, pubmed-meshheading:1651571-Tissue Distribution
pubmed:year
1991
pubmed:articleTitle
Evidence for arsenic as the immunosuppressive component of gallium arsenide.
pubmed:affiliation
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.