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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2006-4-4
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pubmed:abstractText |
Cyclic voltammetry performed at rapid scan rates on cytochrome P450 from Pseudomonas putida (P450CAM) in didodecyldimethylammonium bromide (DDAB) films on graphite electrodes revealed a couple (E) at 830mV (vs Ag/AgCl). E was not significantly observed at scan rates less than 30V/s at room temperature, suggesting that the oxidized species is unstable. The lifetime of E could be prolonged at 4 degrees C, which allowed reversible access to E at scan rates as low as 1V/s. E was found to be sensitive to imidazole in solution and to variations in pH, suggesting that the redox reaction is occurring at the metal center (i.e., Fe(IV/III)). Electrolysis reactions with different P450 substrates revealed that the electrochemically generated high-valent species is able to convert thioanisole to methyl phenyl sulfoxide.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0162-0134
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
519-23
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
Electrochemical generation of a high-valent state of cytochrome P450.
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pubmed:affiliation |
Department of Chemistry, Occidental College, 1600 Campus Road, Los Angeles, CA 91030, USA. udit@oxy.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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