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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1991-9-4
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pubmed:databankReference |
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pubmed:abstractText |
Thymosin beta 4 (T beta 4), a 43-residue peptide of uncertain function, is widely distributed in most tissues of vertebrates. Southern blot analysis demonstrated that the rat genome contains many closely related T beta 4 sequences. Two distinct T beta 4 sequences were isolated by screening a rat genomic DNA library. Analysis of these clones revealed that they represent T beta 4 retroposons; they lack introns and contain stretches of poly(A) sequence at their 3' ends and direct repeats flanking the cDNA-like sequences. In order to isolate a T beta 4 intron-containing gene, DNA fragments that include T beta 4 introns were generated by polymerase chain reaction, using rat genomic DNA as template. Subsequent Southern blot analysis of rat genomic DNA with intron-specific probes demonstrated the presence of a single T beta 4 intron-containing gene. Upon screening the rat genomic DNA library with an intron-specific probe, two overlapping clones of the T beta 4 intron-containing gene were isolated. Further characterization and sequence analysis showed that the rat T beta 4 intron-containing gene has a 2-kilobase pair-long transcription unit containing two introns. The transcription initiation site and putative regulatory elements of the promoter were identified.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
266
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pubmed:geneSymbol |
T&bgr;<down>4</down>
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14256-61
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1650352-Animals,
pubmed-meshheading:1650352-Base Sequence,
pubmed-meshheading:1650352-Blotting, Southern,
pubmed-meshheading:1650352-DNA,
pubmed-meshheading:1650352-DNA Transposable Elements,
pubmed-meshheading:1650352-Introns,
pubmed-meshheading:1650352-Molecular Sequence Data,
pubmed-meshheading:1650352-Polymerase Chain Reaction,
pubmed-meshheading:1650352-RNA, Messenger,
pubmed-meshheading:1650352-Rats,
pubmed-meshheading:1650352-Thymosin,
pubmed-meshheading:1650352-Transcription, Genetic
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pubmed:year |
1991
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pubmed:articleTitle |
Rat thymosin beta 4 gene. Intron-containing gene and multiple retroposons.
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pubmed:affiliation |
Endocrine Unit, Massachusetts General Hospital, Boston.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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