Linked Life Data

16481498

Source:http://linkedlifedata.com/resource/pubmed/id/16481498

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2006-2-16
pubmed:abstractText
The analyses of genome sequences from Rickettsia conorii and R. prowazekii have allowed the identification of five genes encoding autotransporter proteins, including ompA and four genes annotated in the R. prowazekii genome as "surface cell antigen" (sca) genes. Of these, ompA and sca5 (ompB) are known to encode membrane-exposed antigenic proteins playing a major role in the host's immune response, and sca4 encodes a truncated autotransporter protein. In order to study further the phylogeny of the genus Rickettsia, we attempted amplification and sequencing of the sca2 genes from the 20 currently validated Rickettsia species. Sixteen species exhibited a complete sca2 gene, ranging from 2,727 bp in R. bellii to 5,580 bp in R. rhipicephali. R. helvetica and R. canadensis had a split gene, and R. prowazekii and R. typhi had remnant fragments of sca2. We also identified in R. akari, R. prowazekii, and R. typhi a duplication of the sca2 gene. The phylogenetic trees inferred from both the nucleotide and protein sequences of sca2 showed four clusters of rickettsiae, that is, the R. rickettsii, R. massiliae, R. akari, and typhus groups, which were supported by significant bootstrap values.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0077-8923
pubmed:author
pubmed:issnType
Print
pubmed:volume
1063
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
94-9
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Phylogenetic study of Rickettsia species using sequences of the autotransporter protein-encoding gene sca2.
pubmed:affiliation
Unité des Rickettsies, IFR 48, CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
pubmed:publicationType
Journal Article