Source:http://linkedlifedata.com/resource/pubmed/id/16478634
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2006-5-2
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pubmed:abstractText |
We developed a new hybrid nuclear polyhedrosis virus (NPV) bacmid capable of infecting Spodoptera frugiperda, Tricoplusia ni, and Bombyx mori, and B. mori cell lines for producing hybrid recombinant baculovirus that can carry a gene of interest and express it in a broad range of hosts. A GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 fusion gene was expressed successfully in silkworm larvae using this hybrid bacmid. The hybrid NPV bacmid provides an altogether simple and realistically feasible method for large-scale applications using silkworm larvae. It can be easily managed in E. coli, which has no biohazard safety concerns, in addition to the baculovirus-based expression system.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0166-0934
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
134
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
212-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16478634-Animals,
pubmed-meshheading:16478634-Blotting, Western,
pubmed-meshheading:16478634-Bombyx,
pubmed-meshheading:16478634-Cell Line,
pubmed-meshheading:16478634-DNA Helicases,
pubmed-meshheading:16478634-Genes, Viral,
pubmed-meshheading:16478634-Hybridization, Genetic,
pubmed-meshheading:16478634-Insects,
pubmed-meshheading:16478634-Larva,
pubmed-meshheading:16478634-N-Acetylglucosaminyltransferases,
pubmed-meshheading:16478634-Nucleopolyhedrovirus,
pubmed-meshheading:16478634-Protein Engineering,
pubmed-meshheading:16478634-Recombinant Proteins,
pubmed-meshheading:16478634-Transformation, Genetic
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pubmed:year |
2006
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pubmed:articleTitle |
Construction of hybrib Autographa californica nuclear polyhedrosis bacmid by modification of p143 helicase.
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pubmed:affiliation |
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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