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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-2-14
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pubmed:abstractText |
Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against alpha5beta1 integrin (alpha5beta1), but not by one against anti-alphavbeta3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that alpha5beta1 is involved in this adhesion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
580
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1376-82
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16457822-Binding Sites,
pubmed-meshheading:16457822-Cell Adhesion,
pubmed-meshheading:16457822-Cell Line,
pubmed-meshheading:16457822-Chondrocytes,
pubmed-meshheading:16457822-Connective Tissue Growth Factor,
pubmed-meshheading:16457822-Fibronectins,
pubmed-meshheading:16457822-Humans,
pubmed-meshheading:16457822-Immediate-Early Proteins,
pubmed-meshheading:16457822-Integrin alpha5beta1,
pubmed-meshheading:16457822-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:16457822-Membrane Proteins,
pubmed-meshheading:16457822-Protein Binding,
pubmed-meshheading:16457822-Two-Hybrid System Techniques
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pubmed:year |
2006
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pubmed:articleTitle |
CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin alpha5beta1.
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pubmed:affiliation |
Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 5-1 Shikata-cho, 2-chome, Okayama 700-8525, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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