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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-2-3
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pubmed:abstractText |
There is currently no "gold standard" test for the diagnosis of late-stage Chagas' disease. As a result, protection of the blood supply in areas where Chagas' disease is endemic remains problematic. A panel of 709 serum samples from subjects with confirmed Chagas' disease (n = 195), healthy controls (n = 400), and patients with other parasitic diseases (n = 114) was used to assess enzyme-linked immunosorbent assays (ELISAs) based on a concentrated extract of excretory-secretory antigens from either Brazil or Tulahuen strain Trypanosoma cruzi trypomastigotes (total trypomastigote excretory-secretory antigens [TESAs]). The total TESA-based assays had excellent overall sensitivity (100%) and specificity (>94%), except for cross-reactivity with Leishmania-infected sera. In an attempt to increase the specificity of the assay, immunoaffinity chromatography was used to purify the TESA proteins (TESA(IA) proteins). By Western blotting, a series of polypeptide bands with molecular masses ranging from 60 to 220 kDa were recognized by pooled sera positive for Chagas' disease. An ELISA based on TESA(IA) proteins had a slightly lower sensitivity (98.6%) but an improved specificity (100%) compared to the sensitivity and specificity of the total TESA protein-based ELISAs. A 60-kDa polypeptide was identified as a major contributor to the cross-reactivity with Leishmania. These data suggest the need for field validation studies of TESA- and TESA(IA)-based assays in regions where Chagas' disease is endemic.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-10029313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-10858646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-10958475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-11068814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-11337184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-11527823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-11929690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-12088851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-12128048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-12644241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-15071044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-1730508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-2152210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-2520832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-2694219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-3152273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-3934814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-6428245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-7826016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-8257103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-8862574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-8951611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-9215584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-9280332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-9399545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16455872-9924959
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
291-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16455872-Animals,
pubmed-meshheading:16455872-Antibodies, Protozoan,
pubmed-meshheading:16455872-Antigens, Protozoan,
pubmed-meshheading:16455872-Chagas Disease,
pubmed-meshheading:16455872-Chromatography, Affinity,
pubmed-meshheading:16455872-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16455872-Humans,
pubmed-meshheading:16455872-Sensitivity and Specificity,
pubmed-meshheading:16455872-Serologic Tests,
pubmed-meshheading:16455872-Trypanosoma cruzi
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pubmed:year |
2006
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pubmed:articleTitle |
Purified excreted-secreted antigens from Trypanosoma cruzi trypomastigotes as tools for diagnosis of Chagas' disease.
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pubmed:affiliation |
McGill Center for Tropical Diseases, Montreal General Hospital, Room D7-153, Montreal, Quebec, H3G IA4 Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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