| pubmed-article:1645368 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1645368 | lifeskim:mentions | umls-concept:C0035870 | lld:lifeskim |
| pubmed-article:1645368 | lifeskim:mentions | umls-concept:C0348801 | lld:lifeskim |
| pubmed-article:1645368 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:1645368 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:1645368 | lifeskim:mentions | umls-concept:C0441836 | lld:lifeskim |
| pubmed-article:1645368 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:1645368 | pubmed:dateCreated | 1991-6-28 | lld:pubmed |
| pubmed-article:1645368 | pubmed:abstractText | We adapted the polymerase chain reaction (PCR) to detect the noncultivatable group B and C rotaviruses and introduced a simple and convenient technique to purify viral RNA from stool specimens. Double-stranded RNA present in stool extracts was purified by adsorption to hydroxyapatite and was used as the template for reverse transcription and polymerase amplification. Primer pairs specific for group B (gene 8) and group C (gene 6) rotaviruses were selected to amplify group-characteristic sizes of cDNA copies readily identifiable in ethidium bromide-stained agarose gels. These primer pairs were used separately in individual PCR assays or were pooled with a primer pair specific for group A rotavirus (gene 9) in a combined PCR assay for the simultaneous detection of all three rotavirus groups. The method was very sensitive and was used to identify both human and porcine strains of group B and C rotaviruses in stool specimens. A second PCR amplification with internal group-specific primers served to increase further the sensitivity of the test and to confirm the diagnostic results obtained in the first amplification. | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1645368 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1645368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1645368 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1645368 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:1645368 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:CohenJJ | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:AllenJ RJR | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:CaulE OEO | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:McCraeM AMA | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:GlassR IRI | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:Sinarachatana... | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:SaifL JLJ | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:BremontMM | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:GouveaVV | lld:pubmed |
| pubmed-article:1645368 | pubmed:author | pubmed-author:FangZ YZY | lld:pubmed |
| pubmed-article:1645368 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1645368 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:1645368 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1645368 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1645368 | pubmed:pagination | 519-23 | lld:pubmed |
| pubmed-article:1645368 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:meshHeading | pubmed-meshheading:1645368-... | lld:pubmed |
| pubmed-article:1645368 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1645368 | pubmed:articleTitle | Detection of group B and C rotaviruses by polymerase chain reaction. | lld:pubmed |
| pubmed-article:1645368 | pubmed:affiliation | Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333. | lld:pubmed |
| pubmed-article:1645368 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1645368 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1645368 | lld:pubmed |
