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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-6-28
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pubmed:abstractText |
We adapted the polymerase chain reaction (PCR) to detect the noncultivatable group B and C rotaviruses and introduced a simple and convenient technique to purify viral RNA from stool specimens. Double-stranded RNA present in stool extracts was purified by adsorption to hydroxyapatite and was used as the template for reverse transcription and polymerase amplification. Primer pairs specific for group B (gene 8) and group C (gene 6) rotaviruses were selected to amplify group-characteristic sizes of cDNA copies readily identifiable in ethidium bromide-stained agarose gels. These primer pairs were used separately in individual PCR assays or were pooled with a primer pair specific for group A rotavirus (gene 9) in a combined PCR assay for the simultaneous detection of all three rotavirus groups. The method was very sensitive and was used to identify both human and porcine strains of group B and C rotaviruses in stool specimens. A second PCR amplification with internal group-specific primers served to increase further the sensitivity of the test and to confirm the diagnostic results obtained in the first amplification.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-1696283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2155248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2155916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2158190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2165108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2171196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2341836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2479654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2536755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2539512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2547880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2551975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2555422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2852892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-2853177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-3009541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-3016112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-3029161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-3029164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-3034964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-6299944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-6327847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1645368-7041255
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
519-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1645368-Base Sequence,
pubmed-meshheading:1645368-DNA, Viral,
pubmed-meshheading:1645368-Genes, Viral,
pubmed-meshheading:1645368-Humans,
pubmed-meshheading:1645368-Molecular Sequence Data,
pubmed-meshheading:1645368-Polymerase Chain Reaction,
pubmed-meshheading:1645368-RNA, Viral,
pubmed-meshheading:1645368-Rotavirus,
pubmed-meshheading:1645368-Rotavirus Infections,
pubmed-meshheading:1645368-Sensitivity and Specificity
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pubmed:year |
1991
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pubmed:articleTitle |
Detection of group B and C rotaviruses by polymerase chain reaction.
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pubmed:affiliation |
Division of Viral and Rickettsial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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