Linked Life Data

16452305

Source:http://linkedlifedata.com/resource/pubmed/id/16452305

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-2-2
pubmed:abstractText
The chromatin remodeling complex SWI/SNF is known to regulate the transcription of several genes by controlling chromatin structure in an ATP-dependent manner. SWI/SNF contains the Swi2p/Snf2p like ATPases BRG1 or BRM exclusively. We found that the expression of BRM gradually increases and that of BRG1 decreases as liver cells differentiate. Chromatin immunoprecipitation assays revealed that the ATPase subunits of SWI/SNF and tumor suppressor retinoblastoma (RB) family proteins bind to the promoter region of the albumin gene in hepatocytes, and that the replacement of BRG1 with BRM and pRB with p130 at this site occurs over the course of differentiation. Small interfering RNA experiments showed that blocking the expression of BRG1 and BRM in fetal and adult hepatocytes, respectively, causes a reduction in albumin expression. In luciferase reporter assays with a pREP4-based reporter plasmid that forms a chromatin structure, BRG1 showed activity stimulating the expression of the albumin promoter mediated by CCAAT/enhancer-binding protein alpha (C/EBPalpha). This enhancement was facilitated by the RB family members pRB and p130. ATPase assays showed that both pRB and C/EBPalpha proteins directly stimulate the ATPase activity of BRG1. Our findings suggest that the mechanism by which the activity of transcription factors is enhanced by RB family members and SWI/SNF includes an increase in the ATPase activity of the chromatin remodeling complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-88
pubmed:dateRevised
2007-12-19
pubmed:meshHeading
pubmed-meshheading:16452305-Albumins, pubmed-meshheading:16452305-Animals, pubmed-meshheading:16452305-Blotting, Western, pubmed-meshheading:16452305-CCAAT-Enhancer-Binding Protein-beta, pubmed-meshheading:16452305-Cell Differentiation, pubmed-meshheading:16452305-Cells, Cultured, pubmed-meshheading:16452305-Chromosomal Proteins, Non-Histone, pubmed-meshheading:16452305-DNA Helicases, pubmed-meshheading:16452305-Gene Expression Regulation, pubmed-meshheading:16452305-Hepatocytes, pubmed-meshheading:16452305-Liver, pubmed-meshheading:16452305-Mice, pubmed-meshheading:16452305-Nuclear Proteins, pubmed-meshheading:16452305-RNA, Small Interfering, pubmed-meshheading:16452305-Rats, pubmed-meshheading:16452305-Rats, Sprague-Dawley, pubmed-meshheading:16452305-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16452305-Transcription Factors
pubmed:year
2006
pubmed:articleTitle
Mammalian chromatin remodeling complex SWI/SNF is essential for enhanced expression of the albumin gene during liver development.
pubmed:affiliation
Department of Biotechnology, Graduate School of Engineering, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't