Linked Life Data

1644214

Source:http://linkedlifedata.com/resource/pubmed/id/1644214

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1992-9-8
pubmed:abstractText
1. A fucoidan-binding protein from human placenta was purified by affinity chromatography on immobilized fucoidan. 2. Characterization of molecular and immunological properties and peptide mapping indicated that the fucoidan-binding protein is an immunoglobulin G. 3. Cleavage with papain and transblot analysis with labelled fucoidan ascertained binding properties of the F(ab) fragments. 4. The specificity for fucoidan was furthermore substantiated by hapten inhibition of haemagglutination as well as by solid-phase assays with biotinylated fucoidan as ligand. The results emphasized the importance of structural features instead of simple ionic interactions. 5. Chemical modification with group-specific reagents to lysine, arginine, tryptophan, tyrosine and histidine resulted in substantial inactivation, their impact being markedly reduced by the presence of fucoidan in the cases of lysine, arginine and tryptophan.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0020-711X
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1329-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Purification and properties of a fucoidan-binding protein from human placenta and its identification as immunoglobulin G.
pubmed:affiliation
Institut für Pharmazeutische Chemie, Abteilung Glykobiochemie und Angewandte Tumorlektinologie, Marburg, Fed. Rep. Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't