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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-1-20
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pubmed:abstractText |
The kinetics of exo-endocytotic recycling could restrict information transfer at central synapses if neurotransmission were entirely reliant on classical full-collapse fusion. Nonclassical fusion retrieval by kiss-and-run would be kinetically advantageous but remains controversial. We used a hydrophilic quencher, bromophenol blue (BPB), to help detect nonclassical events. Upon stimulation, extracellular BPB entered synaptic vesicles and quenched FM1-43 fluorescence, indicating retention of FM dye beyond first fusion. BPB also quenched fluorescence of VAMP (synaptobrevin-2)-EGFP, thus indicating the timing of first fusion of vesicles in the total recycling pool. Comparison with FM dye destaining revealed that kiss-and-run strongly prevailed over full-collapse fusion at low frequency, giving way to a near-even balance at high frequency. Quickening of kiss-and-run vesicle reuse was also observed at higher frequency in the average single vesicle fluorescence response. Kiss-and-run and reuse could enable hippocampal nerve terminals to conserve scarce vesicular resources when responding to widely varying input patterns.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
243-56
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16423698-Animals,
pubmed-meshheading:16423698-Bromphenol Blue,
pubmed-meshheading:16423698-Cells, Cultured,
pubmed-meshheading:16423698-Coloring Agents,
pubmed-meshheading:16423698-Electric Stimulation,
pubmed-meshheading:16423698-Endocytosis,
pubmed-meshheading:16423698-Exocytosis,
pubmed-meshheading:16423698-Green Fluorescent Proteins,
pubmed-meshheading:16423698-Hippocampus,
pubmed-meshheading:16423698-Kinetics,
pubmed-meshheading:16423698-Neurons,
pubmed-meshheading:16423698-Presynaptic Terminals,
pubmed-meshheading:16423698-Pyridinium Compounds,
pubmed-meshheading:16423698-Quaternary Ammonium Compounds,
pubmed-meshheading:16423698-Rats,
pubmed-meshheading:16423698-Rats, Sprague-Dawley,
pubmed-meshheading:16423698-Synapses,
pubmed-meshheading:16423698-Synaptic Transmission,
pubmed-meshheading:16423698-Temperature
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pubmed:year |
2006
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pubmed:articleTitle |
Frequency-dependent kinetics and prevalence of kiss-and-run and reuse at hippocampal synapses studied with novel quenching methods.
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pubmed:affiliation |
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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