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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2006-3-13
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pubmed:abstractText |
Uracil-DNA glycosylase (UDG) is an enzyme involved in the base excision repair pathway. It specifically removes uracil from both single-stranded and double-stranded DNA. The genome of the Bacillus subtilis phage 29 is a linear double-stranded DNA with a terminal protein covalently linked at each 5'-end. Replication of 29 DNA starts by a protein-priming mechanism and generates intermediates that have long stretches of single-stranded DNA. By using in vivo chemical cross-linking and affinity chromatography techniques, we found that UDG is a cellular target for the early viral protein p56. Addition of purified protein p56 to B. subtilis extracts inhibited the endogenous UDG activity. Moreover, extracts from 29-infected cells were deficient in UDG activity. We suggested that inhibition of the cellular UDG is a defense mechanism developed by 29 to prevent the action of the base excision repair pathway if uracil residues arise in their replicative intermediates. Protein p56 is the first example of a UDG inhibitor encoded by a non-uracil-containing viral DNA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7068-74
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16421108-Bacillus Phages,
pubmed-meshheading:16421108-Bacillus subtilis,
pubmed-meshheading:16421108-Chromatography, Affinity,
pubmed-meshheading:16421108-Cross-Linking Reagents,
pubmed-meshheading:16421108-DNA,
pubmed-meshheading:16421108-DNA, Viral,
pubmed-meshheading:16421108-DNA Glycosylases,
pubmed-meshheading:16421108-DNA Repair,
pubmed-meshheading:16421108-Enzyme Inhibitors,
pubmed-meshheading:16421108-Genome,
pubmed-meshheading:16421108-Genome, Bacterial,
pubmed-meshheading:16421108-Models, Genetic,
pubmed-meshheading:16421108-N-Glycosyl Hydrolases,
pubmed-meshheading:16421108-Protein Binding,
pubmed-meshheading:16421108-Substrate Specificity,
pubmed-meshheading:16421108-Uracil,
pubmed-meshheading:16421108-Uracil-DNA Glycosidase,
pubmed-meshheading:16421108-Viral Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
A uracil-DNA glycosylase inhibitor encoded by a non-uracil containing viral DNA.
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pubmed:affiliation |
Instituto de Biología Molecular "Eladio Viñuela" (Consejo Superior de Investigaciones Científicas), Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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