Source:http://linkedlifedata.com/resource/pubmed/id/16416964
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2006-1-18
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pubmed:abstractText |
To investigate the new method that the primordial germ cells (PGCs) can long time propagate, grow and keep potency of differentiation in vitro. PGCs were co-cultured with Sertoli cells (SCs) or with homologue fibroblasts from genital ridge. When co-cultured with SCs, PGCs colonies and times of transfer of culture were greater than those co-cultured with homologue fibroblasts. At present PGCs of co-cultured with SCs have succeeded in subculturing to the 51 generation. SCs can availably enhance propagating ability as well as a long period of time maintain the characteristic of PGCs.
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pubmed:language |
chi
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0001-5334
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
474-80
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pubmed:meshHeading |
pubmed-meshheading:16416964-Animals,
pubmed-meshheading:16416964-Cell Culture Techniques,
pubmed-meshheading:16416964-Cells, Cultured,
pubmed-meshheading:16416964-Coculture Techniques,
pubmed-meshheading:16416964-Embryonic Stem Cells,
pubmed-meshheading:16416964-Fibroblasts,
pubmed-meshheading:16416964-Germ Cells,
pubmed-meshheading:16416964-Immunohistochemistry,
pubmed-meshheading:16416964-Male,
pubmed-meshheading:16416964-Mice,
pubmed-meshheading:16416964-Sertoli Cells
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pubmed:year |
2005
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pubmed:articleTitle |
[A new culture method for primordial germ cell].
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pubmed:affiliation |
Reproductive Medical Center,Capital University of Medical Sciences, Beijing 100069.
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pubmed:publicationType |
Journal Article,
English Abstract
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