|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2006-1-31
|
|
pubmed:abstractText |
Majority of cancer cells upregulate co-inhibitory molecule B7-H1 which confers resistance to anti-tumor immunity, allowing cancers to escape from host immune surveillance. We addressed the molecular mechanism underlying the regulation of cancer-associated B7-H1 expression in response to interferon-gamma (IFN-gamma). Using promoter constructs in luciferase assay, the region between 202 and 320 bp from the translational start site is responsible for B7-H1 expression. Electrophoretic mobility shift assay, site-directed mutagenesis and knockdown experiment using siRNA revealed that interferon regulatory factor-1 (IRF-1) is primarily responsible for the constitutive B7-H1 expression as well as for the IFN-gamma-mediated B7-H1 upregulation in a human lung cancer cell line A549. Additionally, AG490, a Janus activated kinase/signal transducer and activator of transcription inhibitor, greatly abolished the responsiveness of A549 cells to IFN-gamma by reducing the IRF-1 transcription. Our findings support a critical role of IRF-1 in the regulation of constitutive and IFN-gamma-induced expression of B7-H1 in cancer cells.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD274,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/CD274 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrphostins,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-cyano-(3,4-dihydroxy)-N-benzyl...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
pubmed-author:ChenLiepingL,
pubmed-author:ChoiIn-HakIH,
pubmed-author:JangByeong-ChurlBC,
pubmed-author:LeeSeung-JinSJ,
pubmed-author:LeeSoo-WoongSW,
pubmed-author:OhSangtaekS,
pubmed-author:ParkYeong-MinYM,
pubmed-author:ShinJae-GookJG,
pubmed-author:SuhSeong-IlSI,
pubmed-author:YangYoung-IlYI,
pubmed-author:YaoShengS
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
6
|
|
pubmed:volume |
580
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
755-62
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:16413538-Antigens, CD,
pubmed-meshheading:16413538-Antigens, CD274,
pubmed-meshheading:16413538-Antigens, CD80,
pubmed-meshheading:16413538-Antineoplastic Agents,
pubmed-meshheading:16413538-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16413538-Enzyme Inhibitors,
pubmed-meshheading:16413538-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:16413538-HeLa Cells,
pubmed-meshheading:16413538-Humans,
pubmed-meshheading:16413538-Interferon Regulatory Factor-1,
pubmed-meshheading:16413538-Interferon-gamma,
pubmed-meshheading:16413538-Membrane Glycoproteins,
pubmed-meshheading:16413538-Mutagenesis, Site-Directed,
pubmed-meshheading:16413538-Peptides,
pubmed-meshheading:16413538-Promoter Regions, Genetic,
pubmed-meshheading:16413538-Protein-Tyrosine Kinases,
pubmed-meshheading:16413538-RNA, Small Interfering,
pubmed-meshheading:16413538-Signal Transduction,
pubmed-meshheading:16413538-Transcription, Genetic,
pubmed-meshheading:16413538-Tumor Escape,
pubmed-meshheading:16413538-Tyrphostins,
pubmed-meshheading:16413538-Up-Regulation
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Interferon regulatory factor-1 is prerequisite to the constitutive expression and IFN-gamma-induced upregulation of B7-H1 (CD274).
|
|
pubmed:affiliation |
Department of Microbiology, Inje University College of Medicine, Busan 614-735, Republic of Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|
