Linked Life Data

16413408

Source:http://linkedlifedata.com/resource/pubmed/id/16413408

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-1-17
pubmed:abstractText
Nonphagocytic NADPH oxidases (Noxs) are major sources of reactive oxygen species (ROS) and exist as a family of isoenzymes with tissue-restricted expression and functions. Nox1, expressed in colon epithelium and vascular smooth muscle, is suggested to be involved in innate immune defense and cell growth or proliferation. The transcriptional regulation of Nox1 appears to be particularly important in the modulation of its activity but the underlying mechanisms are unknown. Here we have identified the functional Nox1 promoter in human colon epithelial Caco-2 cells, and show that a 520-bp genomic fragment encompassing the CAP site is sufficient to direct high levels of expression of a linked reporter gene in these cells. Deletion analyses together with electrophoretic mobility-shift assays (EMSAs) suggest that maximal promoter activity is dependent on a GATA-binding site, conserved between human and mouse, within the proximal promoter region. The ability of mouse GATA factors to transactivate the Nox1 promoter was demonstrated in Cos-7 cells and site-directed mutagenesis of the conserved GATA-binding site further demonstrates that the regulation of Nox1 transcription is mediated by the direct binding of a GATA factor to the Nox1 proximal promoter. We also identified more distal, upstream regions which act to repress significantly expression from the Nox1 promoter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0891-5849
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
260-74
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16413408-Amino Acid Sequence, pubmed-meshheading:16413408-Animals, pubmed-meshheading:16413408-COS Cells, pubmed-meshheading:16413408-Caco-2 Cells, pubmed-meshheading:16413408-Cells, Cultured, pubmed-meshheading:16413408-Cercopithecus aethiops, pubmed-meshheading:16413408-Colon, pubmed-meshheading:16413408-Epithelial Cells, pubmed-meshheading:16413408-GATA4 Transcription Factor, pubmed-meshheading:16413408-GATA5 Transcription Factor, pubmed-meshheading:16413408-GATA6 Transcription Factor, pubmed-meshheading:16413408-Gene Expression Regulation, Enzymologic, pubmed-meshheading:16413408-Humans, pubmed-meshheading:16413408-Isoenzymes, pubmed-meshheading:16413408-Molecular Sequence Data, pubmed-meshheading:16413408-NADPH Oxidase, pubmed-meshheading:16413408-Promoter Regions, Genetic, pubmed-meshheading:16413408-RNA, Messenger, pubmed-meshheading:16413408-Transcription, Genetic
pubmed:year
2006
pubmed:articleTitle
Transcriptional regulation of the NADPH oxidase isoform, Nox1, in colon epithelial cells: role of GATA-binding factor(s).
pubmed:affiliation
King's College London, Department of Cardiology, GKT School of Medicine and Dentistry, New Medical School Building, Bessemer Road, London SE5 9PJ, UK. alison.brewer@kcl.ac.uk
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't