Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-12-23
pubmed:abstractText
Many diabetic individuals develop anosmia but the mechanism(s) causing the dysfunction in the olfactory system is (are) unknown. Glial fibrillary acidic protein expression is reduced in diabetic retinopathy and is also reduced, with unknown consequences, in other brain regions of diabetic rats. We used immunohistochemistry and immunoblotting from untreated control and streptozotocin-induced type 1 (insulin dependent) diabetic rats to investigate main olfactory epithelial mitotic rate and glial fibrillary acidic protein expression in the lamina propria of the sensory epithelium and in the olfactory bulb. Numbers of bromodeoxyuridine-positive cells were significantly lower in the diabetic sensory epithelium compared to non-diabetic controls. Immunohistochemical observations suggested a qualitative difference in glial fibrillary acidic protein expression in both regions examined especially in the olfactory bulb external plexiform layer and the lamina propria. Immunoblot analysis confirmed that the diabetic olfactory bulb and lamina propria expressed less glial fibrillary acidic protein compared to the non-diabetic control group. The lower expression levels in the olfactory bulb external plexiform layer suggested by immunohistochemistry do not reflect a change in the number of astrocytes since the numbers of S100B(+) cells were not different between the two groups.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0300-4864
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3-10
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16374706-Animals, pubmed-meshheading:16374706-Cell Count, pubmed-meshheading:16374706-Cytoskeleton, pubmed-meshheading:16374706-Diabetes Mellitus, Experimental, pubmed-meshheading:16374706-Diabetic Neuropathies, pubmed-meshheading:16374706-Fluorescent Antibody Technique, pubmed-meshheading:16374706-Glial Fibrillary Acidic Protein, pubmed-meshheading:16374706-Immunoblotting, pubmed-meshheading:16374706-Immunohistochemistry, pubmed-meshheading:16374706-Male, pubmed-meshheading:16374706-Mitosis, pubmed-meshheading:16374706-Mucous Membrane, pubmed-meshheading:16374706-Nasal Bone, pubmed-meshheading:16374706-Olfaction Disorders, pubmed-meshheading:16374706-Olfactory Bulb, pubmed-meshheading:16374706-Olfactory Mucosa, pubmed-meshheading:16374706-Rats, pubmed-meshheading:16374706-Rats, Wistar
pubmed:year
2005
pubmed:articleTitle
Changes in mitotic rate and GFAP expression in the primary olfactory axis of streptozotocin-induced diabetic rats.
pubmed:affiliation
Department of Anatomy, Physiology, Pharmacology, College of Veterinary Medicine, Auburn University, AL 36849-0551, USA. dennijc@vetmed.auburn.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural