Linked Life Data

16368064

Source:http://linkedlifedata.com/resource/pubmed/id/16368064

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-12-21
pubmed:abstractText
Suppressor of cytokine signaling 3 (SOCS3) was reported as a feedback inhibitor of cytokine receptor signaling by inhibiting the JAK-STAT signal transduction pathway. We sought to test the anti-endotoxic septic shock effect of liposome mediated gene delivery of SOCS3 in a lethal endotoxic shock mouse model. BALB/c mice were injected intraperitoneally with 200 microg pcDNA3.1-SOCS3 cationic liposomes, while pcDNA3.1-IL-10 and empty vector as positive and negative control respectively. Forty-eight hours after gene delivery, mice were challenged with 4 microg of E.coli 0127:B8 LPS and 18 mg D-GalN administered i.p. 90 min later, serum TNF-alpha level was determined. Survival over the next 48 h was evaluated. Peritoneal macrophages from survival mice were stimulated in vitro with 1 ug/ml LPS for 18 h, and the supernatants were harvested for determination of the amount of TNF-alpha. We found that gene delivery of SOCS3 significantly increase the mouse survival rate from 27.8 +/- 9.6% of control group to 61.1 +/- 9.6% (p < 0.01). In comparison with control group (218 +/- 13 pg/ml) and sham delivery group (2,122 pg/ml), gene delivery of SOCS3 reduced the level of serum TNF-alpha (68 +/- 9 pg/ml) significantly (p < 0.01). Furthermore, gene delivery of SOCS3 displayed the capacity of prevention of tolerance of peritoneal macrophages to LPS. These findings suggest that gene delivery of SOCS3 mediated by liposome is a promising approach for endotoxic septic shock treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1672-7681
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
373-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Gene delivery of SOCS3 protects mice from lethal endotoxic shock.
pubmed:affiliation
Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. minfang89@yahoo.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't