Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2005-12-19
pubmed:abstractText
Array-based comparative genomic hybridization is a high resolution method for measuring chromosomal copy number changes. Here we present a validated protocol using in-house spotted oligonucleotide libraries for array comparative genomic hybridization (CGH). This oligo array CGH platform yields reproducible results and is capable of detecting single copy gains, multi-copy amplifications as well as homozygous and heterozygous deletions as small as 100 kb with high resolution. A human oligonucleotide library was printed on amine binding slides. Arrays were hybridized using a hybstation and analysed using BlueFuse feature extraction software, with >95% of spots passing quality control. The protocol allows as little as 300 ng of input DNA and a 90% reduction of Cot-1 DNA without compromising quality. High quality results have also been obtained with DNA from archival tissue. Finally, in addition to human oligo arrays, we have applied the protocol successfully to mouse oligo arrays. We believe that this oligo-based platform using 'off-the-shelf' oligo libraries provides an easy accessible alternative to BAC arrays for CGH, which is cost-effective, available at high resolution and easily implemented for any sequenced organism without compromising the quality of the results.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-10471496, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-10748872, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-11687795, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-1359641, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-14707179, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-14981516, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15166273, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15201182, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15256441, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15273396, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15521975, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15591353, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15640221, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15681476, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15687294, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15756638, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15800641, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15832308, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-15920524, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-16081473, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-16159913, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-16288008, http://linkedlifedata.com/resource/pubmed/commentcorrection/16361265-9771718
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1362-4962
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e192
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Human and mouse oligonucleotide-based array CGH.
pubmed:affiliation
Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies