Source:http://linkedlifedata.com/resource/pubmed/id/16353238
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2006-1-25
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| pubmed:abstractText |
Oxidative stress has been suggested to be an important mediator of dopaminergic cell death in Parkinson's disease (PD). We investigated the neuroprotective potential of Cu/Zn superoxide dismutase (SOD1) overexpression in the rat substantia nigra (SN) following adenovirus-mediated gene transfer. Human dopaminergic SK-N-SH cells were transduced with adenoviral vectors expressing either human SOD1 (Ad-SOD1) or beta-galactosidase (Ad-betagal) before exposure to 1 mM of the 1-methyl-4-phenylpyridinium ion (MPP+). A strong neuroprotective effect of SOD1 gene transfer was observed in the SK-N-SH cells exposed to MPP+ compared with controls. Adult rats were then given unilateral injections of either Ad-SOD1 or Ad-betagal into the striatum, and MPP+ was administered 8 days later at the same location. Strong transgene expression was detected in the SN dopaminergic neurons, a consequence of retrograde axonal transport of the adenoviral particles. The amphetamine-induced rotational behavior of the rats was markedly lower in Ad-SOD1-injected rats than in control animals. Also, behavioral recovery significantly correlated with the number of tyrosine hydrolase-expressing neurons in the SN of the treated rats. These results are consistent with oxidative stress contributing to the MPP+ -induced neurodegenerative process. They also indicate that SOD1 gene transfer into the nigrostriatal system may be a potential neuroprotective strategy for treating PD.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-4-phenylpyridinium,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0360-4012
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2005 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
83
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
233-42
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16353238-1-Methyl-4-phenylpyridinium,
pubmed-meshheading:16353238-Animals,
pubmed-meshheading:16353238-Cell Count,
pubmed-meshheading:16353238-Cell Line, Tumor,
pubmed-meshheading:16353238-Disease Models, Animal,
pubmed-meshheading:16353238-Female,
pubmed-meshheading:16353238-Gene Therapy,
pubmed-meshheading:16353238-Gene Transfer Techniques,
pubmed-meshheading:16353238-Genetic Vectors,
pubmed-meshheading:16353238-Humans,
pubmed-meshheading:16353238-Immunohistochemistry,
pubmed-meshheading:16353238-Motor Activity,
pubmed-meshheading:16353238-Neuroblastoma,
pubmed-meshheading:16353238-Neurotoxicity Syndromes,
pubmed-meshheading:16353238-Parkinson Disease,
pubmed-meshheading:16353238-Rats,
pubmed-meshheading:16353238-Rotarod Performance Test,
pubmed-meshheading:16353238-Substantia Nigra,
pubmed-meshheading:16353238-Superoxide Dismutase,
pubmed-meshheading:16353238-Tetrazolium Salts,
pubmed-meshheading:16353238-Thiazoles,
pubmed-meshheading:16353238-Transgenes,
pubmed-meshheading:16353238-Tyrosine 3-Monooxygenase
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| pubmed:year |
2006
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| pubmed:articleTitle |
1-methyl-4-phenylpyridinium neurotoxicity is attenuated by adenoviral gene transfer of human Cu/Zn superoxide dismutase.
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| pubmed:affiliation |
Laboratoire de Genetique Moleculaire de la Neurotransmission et des Processus Neurodegeneratifs (LGN), CNRS UMR 7091, Paris, France. barkats@genethon.fr
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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