Linked Life Data

16349705

Source:http://linkedlifedata.com/resource/pubmed/id/16349705

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-6-25
pubmed:abstractText
A rapid sensitive method for the detection of viable bacterial cells is described in which P as inorganic orthophosphate is used to label the cells. Factors affecting the uptake of P by cells as well as the sensitivity of the method have been explored with suspensions of Aerobacter aerogenes. The uptake of PO(4) is dependent on several factors. Of various incubation media tested, one composed of 0.005 m KCl, 0.002 m MgSO(4) and 10 mg/ml of glucose was found to best stimulate the uptake of the tracer. Incubation time and temperature and level of isotope and of unlabeled P also affected uptake. Labeled cells were collected on a membrane filter for measurement of radioactivity. Under optimal conditions, as few as 23 viable cells per milliliter were detected in 1 hr with 95% confidence.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Nov
pubmed:issn
0003-6919
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
979-84
pubmed:dateRevised
2010-9-20
pubmed:year
1966
pubmed:articleTitle
Sensitive rapid detection method for viable bacterial cells.
pubmed:affiliation
Department of Microbiology, Macdonald College of McGill University, Ottawa, Ontario, Canada.
pubmed:publicationType
Journal Article