Source:http://linkedlifedata.com/resource/pubmed/id/16345389
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2010-6-25
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| pubmed:abstractText |
Phanerochaete chrysosporium produces intracellular soluble and particulate beta-glucosidases and an extracellular beta-glucosidase. The extracellular enzyme is induced by cellulose but repressed in the presence of glucose. The molecular weight of this enzyme is 90,000. The K(m) for p-nitrophenyl-beta-glucoside is 1.6 x 10 M; the K(i) for glucose, a competitive inhibitor, is 5.0 x 10 M. The K(m) for cellobiose is 5.3 x 10 M. The intracellular soluble enzyme is induced by cellobiose; this induction is prevented by cycloheximide. The presence of 300 mM glucose in the medium, however, had no effect on induction. The K(m) for p-nitrophenyl-beta-glucoside is 1.1 x 10 M. The molecular weight of this enzyme is approximately 410,000. Both enzymes have an optimal temperature of 45 degrees C and an E(act) of 9.15 kcal (ca. 3.83 x 10 J). The pH optima, however, were approximately 7.0 and 5.5 for the intracellular and extracellular enzymes, respectively.
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| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-101374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-1058470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-14233912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-407476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-4854948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-5062915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-5647272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-5812970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-5862401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-6049589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16345389-97
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0099-2240
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
37
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
938-42
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| pubmed:dateRevised |
2010-9-20
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| pubmed:year |
1979
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| pubmed:articleTitle |
Phanerochaete chrysosporium beta-Glucosidases: Induction, Cellular Localization, and Physical Characterization.
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| pubmed:affiliation |
Department of Chemistry and Biochemical Sciences, Oregon Graduate Center, Beaverton, Oregon 97005.
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| pubmed:publicationType |
Journal Article
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