Linked Life Data

16342763

Source:http://linkedlifedata.com/resource/pubmed/id/16342763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2005-12-13
pubmed:abstractText
To explore a new approach of expression of ABP-CM4 in methylotrophic yeast P. pastoris. The gene of ABP-CM4 was cloned into the vector pPIC9. The Sac I -linearized plasmid pPIC9-CM4 was transformed into P. pastoris GS115 by electroporation. By means of MM and MD plates and PCR, the recombinant P. pastoris strains (his- mut+) were obtained. Antibacterial assay, Tricine-SDS-PAGE and Polyarylamide Gel Electrophoresis demonstrated that ABP-CM4 was extracellularly expressed in P. pastoris with the induction of methanol. The recombinant ABP-CM4 has the antifungal activity on Aspergillus niger and Trichoderma viride.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0001-6209
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
720-3
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
[Cloning, expression and characterization of antibacterial peptide CM4 in Pichia pastoris].
pubmed:affiliation
Jiangsu Province Key Laboratory of Biochemistry and Molecular Biology, College of Life Science, Nanjing Normal University, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't