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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2005-12-12
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pubmed:abstractText |
Peroxiredoxins (Prxs) are a ubiquitous family of peroxidases widely distributed among prokaryotes and eukaryotes. Here, we report on the cloning and functional characterization of a cDNA designated PcPrx-1, encoding peroxiredoxin from the white-rot fungus Phanerochaete chrysosporium. The full-length PcPrx-1 cDNA (932 bp) contains an open reading frame of 200 amino acid residues with a molecular mass of 22.1 kDa. The deduced primary structure of PcPrx-1 polypeptide shows a high level of sequence identity to other recently identified 2-cys peroxiredoxins. The recombinant PcPrx-1 protein was expressed as a histidine fusion protein in Escherichia coli and purified with a Ni2+-column. The purified protein was shown to have a protective effect against plasmid DNA cleavage by reactive oxygen species. The PcPrx-1 protein displays the ability to remove H2O2 in a ferrithiocyanate system. The results of this study suggest that PcPrx-1 may play a protective role against oxidative stress in P. chrysosporium.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1425-8153
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
659-68
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16341274-Amino Acid Sequence,
pubmed-meshheading:16341274-Antioxidants,
pubmed-meshheading:16341274-Base Sequence,
pubmed-meshheading:16341274-Biological Assay,
pubmed-meshheading:16341274-Cloning, Molecular,
pubmed-meshheading:16341274-DNA, Complementary,
pubmed-meshheading:16341274-DNA, Fungal,
pubmed-meshheading:16341274-Escherichia coli,
pubmed-meshheading:16341274-Hydrogen Peroxide,
pubmed-meshheading:16341274-Molecular Sequence Data,
pubmed-meshheading:16341274-Oxidation-Reduction,
pubmed-meshheading:16341274-Peroxidases,
pubmed-meshheading:16341274-Peroxiredoxins,
pubmed-meshheading:16341274-Phanerochaete,
pubmed-meshheading:16341274-Recombinant Proteins,
pubmed-meshheading:16341274-Sequence Analysis, DNA,
pubmed-meshheading:16341274-Sequence Homology, Amino Acid
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pubmed:year |
2005
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pubmed:articleTitle |
Molecular cloning and characterization of a peroxiredoxin from Phanerochaete chrysosporium.
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pubmed:affiliation |
College of Life Sciences, Sichuan University, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, P.R. China. jiangquan@blazemail.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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