Linked Life Data

1632812

Source:http://linkedlifedata.com/resource/pubmed/id/1632812

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1992-8-14
pubmed:abstractText
This study was undertaken to investigate the effect of dietary lipid on the regulation of several constitutive P450 isozymes. Male Sprague-Dawley rats with body weights of 130-140 g were fed either a 20% corn oil (CO) diet or a fat-free (FF) diet for 4 days following 2 days of fasting. Using liver microsomes, the catalytic activities and immunochemically detectable protein levels of P450s 1A1 and 2, 2A1, 2B1 and 2, 2C11, 2E1, and 3A were determined. The microsomes from rats fed the 20% CO diet exhibited 2-fold higher levels in N-nitrosodimethylamine demethylase activity and P450 2E1 protein than those from rats fed the FF diet. The CO group also showed 2.5-fold higher levels in 6 beta-hydroxylation of testosterone and P450 3A protein than the FF group. In contrast, the CO diet did not affect the immunodetectable level of P450 2C11 protein and its catalytic activities such as benzphetamine demethylase activity and 2 alpha-hydroxylation of testosterone. P450 1A1 was not detectable in either group, but 1A2 was 2.5-fold higher in the CO group than in the FF group. In the liver, the P450 2B1 level was very low in both groups as measured by pentoxyresorufin dealkylase activity and the protein level, whereas 2B2 was 2.5-fold higher in the CO diet group. In lung microsomes from rats fed different amounts of CO, an inverse relationship was observed between the P450 2B1 level and the dietary CO level. The results suggest that the constitutive levels of P450 isozymes are modulated by dietary lipid in a selective manner; the levels of hepatic P450s 1A2, 2B2, 2E1, and 3A were regulated positively but the level of pulmonary P450 2B1 was suppressed by dietary lipid.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-2952
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2535-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1632812-Animals, pubmed-meshheading:1632812-Blotting, Western, pubmed-meshheading:1632812-Corn Oil, pubmed-meshheading:1632812-Cytochrome P-450 CYP2B1, pubmed-meshheading:1632812-Cytochrome P-450 CYP2E1, pubmed-meshheading:1632812-Cytochrome P-450 Enzyme System, pubmed-meshheading:1632812-Dietary Fats, pubmed-meshheading:1632812-Isoenzymes, pubmed-meshheading:1632812-Liver, pubmed-meshheading:1632812-Lung, pubmed-meshheading:1632812-Male, pubmed-meshheading:1632812-Microsomes, Liver, pubmed-meshheading:1632812-Models, Biological, pubmed-meshheading:1632812-Nitrosamines, pubmed-meshheading:1632812-Oxidoreductases, pubmed-meshheading:1632812-Oxidoreductases, N-Demethylating, pubmed-meshheading:1632812-Rats, pubmed-meshheading:1632812-Rats, Inbred Strains, pubmed-meshheading:1632812-Testosterone
pubmed:year
1992
pubmed:articleTitle
Modulation of the levels of cytochromes P450 in rat liver and lung by dietary lipid.
pubmed:affiliation
Department of Chemical Biology and Pharmacognosy, College of Pharmacy, Rutgers University, Piscataway, NJ 08855-0789.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.