rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-8-20
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pubmed:abstractText |
The adenosine receptors in the plasma membrane of chromaffin cells from bovine adrenal medulla were characterized. The presence of A1 receptors was discounted owing to the absence of R-[3H]phenylisopropyladenosine (R-PIA) and [3H]8-cyclopentyl-1,3-dipropylxanthine ([3H]-DPCPX) binding. The binding of the specific A2a ligand CGS-21680 was low. In contrast, the binding of 5'-(N-[3H]-ethylcarboxamidoadenosine ([3H]NECA) was relatively high (1.7 pmol/mg of protein at a ligand concentration up to 90 nM). This binding did not correspond to non-adenosine receptor NECA binding sites because the specific [3H]-NECA binding was similar when unlabeled adenosine, NECA, or R-PIA was used to measure the nonspecific binding. The rank order of potency of different ligands for the displacement of specific [3H]NECA binding was DPCPX greater than NECA greater than chloroadenosine greater than R-PIA greater than theophylline = CGS-21680. These results indicate that the receptors present on the plasma membrane of chromaffin cells are exclusively of the A2b subtype.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,3-dipropyl-8-cyclopentylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/2-Chloroadenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine-5'-(N-ethylcarboxamide),
http://linkedlifedata.com/resource/pubmed/chemical/Phenylisopropyladenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Xanthines
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
425-31
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:1629718-2-Chloroadenosine,
pubmed-meshheading:1629718-Adenosine,
pubmed-meshheading:1629718-Adenosine-5'-(N-ethylcarboxamide),
pubmed-meshheading:1629718-Animals,
pubmed-meshheading:1629718-Cattle,
pubmed-meshheading:1629718-Cell Fractionation,
pubmed-meshheading:1629718-Cell Membrane,
pubmed-meshheading:1629718-Chromaffin System,
pubmed-meshheading:1629718-Phenylisopropyladenosine,
pubmed-meshheading:1629718-Radioligand Assay,
pubmed-meshheading:1629718-Receptors, Purinergic,
pubmed-meshheading:1629718-Tritium,
pubmed-meshheading:1629718-Vasodilator Agents,
pubmed-meshheading:1629718-Xanthines
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pubmed:year |
1992
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pubmed:articleTitle |
The adenosine receptors present on the plasma membrane of chromaffin cells are of the A2b subtype.
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pubmed:affiliation |
Departament de Bioquimica i Fisiologia, Unitat de Bioquimica i Biologia Molecular A. Martí i Franquès 1, Barcelona, Spain.
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pubmed:publicationType |
Journal Article
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